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Description
The problem of supporting level 2 moclo designs can be summarized as follows:
- determining appropriate backbones for TU inside MD such that the product of level 1 assembly fits into a level 2 finished plasmid
- 4 TU design will take L1 plasmids with fusion sites AE, EF, FG, GH and backbone DVA_AH
- 3 TU can take AE, EF, FG and backbone DVA_AG
- 2 TU can take AE, EF and backbone DVA_AF etc
- iterating through existing digestion-ligation simulation code with outputs of previous steps as the inputs to the next
inputs = module definition with >1 floating TUs
- add MoClo level attribute to mocloplasmid class
- add antibiotic resistance level to mocloplasmid class
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