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level 2 moclo support #29

@doublergreer

Description

@doublergreer

The problem of supporting level 2 moclo designs can be summarized as follows:

  1. determining appropriate backbones for TU inside MD such that the product of level 1 assembly fits into a level 2 finished plasmid
    • 4 TU design will take L1 plasmids with fusion sites AE, EF, FG, GH and backbone DVA_AH
    • 3 TU can take AE, EF, FG and backbone DVA_AG
    • 2 TU can take AE, EF and backbone DVA_AF etc
  2. iterating through existing digestion-ligation simulation code with outputs of previous steps as the inputs to the next

inputs = module definition with >1 floating TUs

  • add MoClo level attribute to mocloplasmid class
  • add antibiotic resistance level to mocloplasmid class

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