diff --git a/.github/workflows/install_nextflow_v24.10.5.sh b/.github/workflows/install_nextflow_v24.10.5.sh new file mode 100644 index 0000000..2986717 --- /dev/null +++ b/.github/workflows/install_nextflow_v24.10.5.sh @@ -0,0 +1,511 @@ +#!/usr/bin/env bash +# +# Copyright 2013-2024, Seqera Labs +# +# Licensed under the Apache License, Version 2.0 (the "License"); +# you may not use this file except in compliance with the License. +# You may obtain a copy of the License at +# +# http://www.apache.org/licenses/LICENSE-2.0 +# +# Unless required by applicable law or agreed to in writing, software +# distributed under the License is distributed on an "AS IS" BASIS, +# WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied. +# See the License for the specific language governing permissions and +# limitations under the License. + +[[ "$NXF_DEBUG" == 'x' ]] && set -x +NXF_VER=${NXF_VER:-'24.10.5'} +NXF_ORG=${NXF_ORG:-'nextflow-io'} +NXF_HOME=${NXF_HOME:-$HOME/.nextflow} +NXF_PROT=${NXF_PROT:-'https'} +NXF_BASE=${NXF_BASE:-$NXF_PROT://www.nextflow.io/releases} +NXF_TEMP=${NXF_TEMP:-$TMPDIR} +NXF_DIST=${NXF_DIST:-$NXF_HOME/framework} +NXF_CLI="$0 $@" +NXF_CLI_OPTS=${NXF_CLI_OPTS:-} +NXF_REMOTE_DEBUG_PORT=${NXF_REMOTE_DEBUG_PORT:-5005} + +function cmp_ver() { + local IFS=. + local i ver1 ver2 + read -r -a ver1 <<< "${1//./ }" + read -r -a ver2 <<< "${2//./ }" + + # Compare major, minor, and patch numbers + for ((i=0; i<3; i++)); do + ver1[i]=${ver1[i]//[^0-9]} + ver2[i]=${ver2[i]//[^0-9]} + [[ ${ver1[i]:-0} -lt ${ver2[i]:-0} ]] && echo -1 && return + [[ ${ver1[i]:-0} -gt ${ver2[i]:-0} ]] && echo 1 && return + done + + # Extract suffixes for comparison + local suffix1="${1##*.}" + local suffix2="${2##*.}" + suffix1="${suffix1//[0-9]}" + suffix2="${suffix2//[0-9]}" + + # Compare suffixes + [[ -z $suffix1 && -n $suffix2 ]] && echo 1 && return + [[ -n $suffix1 && -z $suffix2 ]] && echo -1 && return + [[ $suffix1 < $suffix2 ]] && echo -1 && return + [[ $suffix1 > $suffix2 ]] && echo 1 && return + + # Versions are equal + echo 0 +} + +# if the nextflow version is greater or equals to "24.07.0-edge" the new shadow jar launcher +# should be used, otherwise fallback on the legacy behavior setting the variable NXF_LEGACY_LAUNCHER +NXF_LEGACY_LAUNCHER=1 +if [[ $(cmp_ver "$NXF_VER" "24.07.0-edge") -ge 0 ]]; then + unset NXF_LEGACY_LAUNCHER +fi + +export NXF_CLI +export NXF_ORG +export NXF_HOME + +if [[ $TERM && $TERM != 'dumb' ]]; then +if command -v tput &>/dev/null; then +GREEN=$(tput setaf 2; tput bold) +YELLOW=$(tput setaf 3) +RED=$(tput setaf 1) +NORMAL=$(tput sgr0) +fi +fi + +function echo_red() { + >&2 echo -e "$RED$*$NORMAL" +} + +function echo_green() { + echo -e "$GREEN$*$NORMAL" +} + +function echo_yellow() { + >&2 echo -e "$YELLOW$*$NORMAL" +} + +function die() { + echo_red "$*" + exit 1 +} + +function get_abs_filename() { + echo "$(cd "$(dirname "$1")" && pwd)/$(basename "$1")" +} + +function get() { + if command -v curl &>/dev/null; then + GET="curl -fsSL '$1' -o '$2'" + elif command -v wget &>/dev/null; then + GET="wget '$1' -O '$2' >/dev/null 2>&1" + else + echo_red "ERROR: Cannot find 'curl' nor 'wget' utility -- please install one of them" + exit 1 + fi + + printf "Downloading nextflow dependencies. It may require a few seconds, please wait .. " + eval $GET; status=$? + printf "\r\033[K" + if [ $status -ne 0 ]; then + echo_red "ERROR: Cannot download nextflow required file -- make sure you can connect to the internet" + echo "" + echo "Alternatively you can try to download this file:" + echo " $1" + echo "" + echo "and save it as:" + echo " ${3:-$2}" + echo "" + exit 1 + fi +} + +function get_ver() { + if command -v curl &>/dev/null; then + curl -fsSL "$1" + elif command -v wget &>/dev/null; then + wget "$1" >/dev/null 2>&1 + else + echo_red "ERROR: Cannot find 'curl' nor 'wget' utility -- please install one of them" + exit 1 + fi +} + +function make_temp() { + local base=${NXF_TEMP:=$PWD} + if [ "$(uname)" = 'Darwin' ]; then mktemp "${base}/nxf-tmp.XXXXXX" || exit $? + else mktemp -t nxf-tmp.XXXXXX -p "${base}" || exit $? + fi +} + +function resolve_link() { + [[ ! -f $1 ]] && exit 1 + if command -v realpath &>/dev/null; then + realpath "$1" + elif command -v readlink &>/dev/null; then + local target="$1" + cd "$(dirname "$target")"; target="$(basename "$target")" + while [ -L "$target" ]; do + target="$(readlink "$target")" + cd "$(dirname "$target")"; target="$(basename "$target")" + done + echo "$(cd "$(dirname "$target")"; pwd -P)/$target" + else + echo_yellow "WARN: Neither \`realpath\` nor \`readlink\` command can be found" + exit 1 + fi +} + +function current_ver() { + [[ $NXF_EDGE == 1 || $NXF_VER == *"-edge" ]] && printf 'edge' || printf 'latest' +} + +function install() { + local tmpfile=$(make_temp) + local version=$(set +u; [[ $NXF_VER ]] && printf "v$NXF_VER" || current_ver) + local action="a=${2:-default}" + get "$NXF_BASE/$version/nextflow?$action" "$tmpfile" "$1" || exit $? + mv "$tmpfile" "$1" || exit $? + chmod +x "$1" || exit $? + bash "$1" -download || exit $? + echo '' + echo -e $'Nextflow installation completed. Please note:' + echo -e $'- the executable file `nextflow` has been created in the folder:' $(dirname $1) + if [[ ! "$PATH" =~ (^|:)"$(dirname $1)"(:|$) ]]; then + echo -e $'- you may complete the installation by moving it to a directory in your $PATH' + fi + echo '' +} + +function check_latest() { + [[ $cmd != run ]] && return 0 + [[ $NXF_OFFLINE == true || $NXF_DISABLE_CHECK_LATEST == true ]] && return 0 + local latest=$(get_ver "$NXF_BASE/$(current_ver)/version?current=$NXF_VER") + if [[ -n "$latest" && $(cmp_ver "$latest" "$NXF_VER") -gt 0 ]]; then + echo_yellow "Nextflow $latest is available - Please consider updating your version to it" + fi +} + +function launch_nextflow() { + # the launch command line + local cmdline=() + # remove leading and trailing double-quotes + for x in "${launcher[@]}"; do + x="${x%\"}" + x="${x#\"}" + cmdline+=("$x") + done + + if [[ "$bg" ]]; then + local pid_file="${NXF_PID_FILE:-.nextflow.pid}" + cmdline+=("${args[@]}") + exec "${cmdline[@]}" & + disown + echo $! > "$pid_file" + exit 0 + fi + + cmdline+=("${args[@]}") + exec "${cmdline[@]}" + exit 1 +} + +# check self-install +if [ "$0" = "bash" ] || [[ "$0" =~ .*/bash ]]; then + if [ -d nextflow ]; then + echo 'Please note:' + echo "- The install procedure needs to create a file named 'nextflow' in this folder, but a directory with this name already exists." + echo "- Please renamed/delete that directory, or execute the Nextflow install procedure in another folder." + echo '' + exit 1 + fi + install "$PWD/nextflow" install + exit 0 +fi + +# clean up env +# see https://github.com/nextflow-io/nextflow/issues/1716 +unset JAVA_TOOL_OPTIONS + +# parse the command line +bg='' +declare -a jvmopts=() +declare -a args=("$@") +declare -a commands=(clone config drop help history info ls pull run view node console kuberun) +# $NXF_CLI_OPTS allow to pass arbitrary cli opts via the environment +# note: do not wrap with quotes because the variable can be used to specify more than on option separate by blanks +[ "$NXF_CLI_OPTS" ] && args+=($NXF_CLI_OPTS) + +cmd='' +while [[ $# != 0 ]]; do + case $1 in + -D*) + if [[ ! "$cmd" ]]; then + jvmopts+=("$1") + fi + ;; + -bg) + bg=1 + ;; + -remote-debug) + echo_yellow "Enabling script debugging - continue the execution launching the remote VM debugger in your favourite IDE using port $NXF_REMOTE_DEBUG_PORT" + remote_debug=1 + ;; + -download) + if [[ ! "$cmd" ]]; then + rm -rf "$NXF_DIST/$NXF_VER" || exit $? + bash "$0" -version || exit $? + exit 0 + fi + ;; + -self-update|self-update) + if [[ ! "$cmd" ]]; then + [[ -z $NXF_EDGE && $NXF_VER = *-edge ]] && NXF_EDGE=1 + unset NXF_VER + install "$0" update + exit 0 + fi + ;; + *) + [[ $1 && $1 != -* && ! "$cmd" && ${commands[*]} =~ $1 ]] && cmd=$1 + ;; + esac + shift +done + +CAPSULE_LOG=${CAPSULE_LOG:=''} +CAPSULE_RESET=${CAPSULE_RESET:=''} +CAPSULE_CACHE_DIR=${CAPSULE_CACHE_DIR:="$NXF_HOME/capsule"} + +NXF_PACK=one +NXF_MODE=${NXF_MODE:-''} +NXF_JAR=${NXF_JAR:-nextflow-$NXF_VER-$NXF_PACK.jar} +NXF_BIN=${NXF_BIN:-$NXF_DIST/$NXF_VER/$NXF_JAR} +NXF_PATH=$(dirname "$NXF_BIN") +NXF_URL=${NXF_URL:-$NXF_BASE/v$NXF_VER/$NXF_JAR} +NXF_HOST=${HOSTNAME:-localhost} +[[ $NXF_LAUNCHER ]] || NXF_LAUNCHER=${NXF_HOME}/tmp/launcher/nextflow-${NXF_PACK}_${NXF_VER}/${NXF_HOST} +# both NXF_GRAB and NXF_CLASSPATH are not supported any more as of version 24.04.7-edge +NXF_GRAB=${NXF_GRAB:-''} +NXF_CLASSPATH=${NXF_CLASSPATH:-''} + +# Determine the path to this file +if [[ $NXF_PACK = dist ]]; then + NXF_BIN=$(which "$0" 2>/dev/null) + [ $? -gt 0 -a -f "$0" ] && NXF_BIN="./$0" +fi + +# use nextflow custom java home path +if [[ "$NXF_JAVA_HOME" ]]; then + JAVA_HOME="$NXF_JAVA_HOME" + unset JAVA_CMD +fi +# Determine the Java command to use to start the JVM. +if [ ! -x "$JAVA_CMD" ] ; then + if [ -d "$JAVA_HOME" ] ; then + if [ -x "$JAVA_HOME/jre/sh/java" ] ; then + # IBM's JDK on AIX uses strange locations for the executables + JAVA_CMD="$JAVA_HOME/jre/sh/java" + else + JAVA_CMD="$JAVA_HOME/bin/java" + fi + elif [ -x /usr/libexec/java_home ]; then + JAVA_CMD="$(/usr/libexec/java_home -v 11+ 2>/dev/null)/bin/java" || JAVA_CMD=java + else + JAVA_CMD="$(which java)" || JAVA_CMD=java + fi +fi + +# Retrieve the java version from a NF local file +JAVA_KEY="$NXF_HOME/tmp/ver/$(resolve_link "$JAVA_CMD" | sed 's@/@.@g')" +if [ -f "$JAVA_KEY" ]; then + JAVA_VER="$(cat "$JAVA_KEY")" +else + JAVA_VER="$("$JAVA_CMD" $NXF_OPTS -version 2>&1)" + if [ $? -ne 0 ]; then + getstarted_web="https://www.nextflow.io/docs/latest/getstarted.html" + echo_red "${JAVA_VER:-Failed to launch the Java virtual machine}" + echo_red "NOTE: Nextflow needs a Java virtual machine to run. To this end: + - make sure a \`java\` command can be found; or + - manually define the variables JAVA_HOME to point to an existing installation; or + - install a Java virtual machine, for instance through https://sdkman.io (read the docs); + - for more details please refer to the Nextflow Get Started page at http://docs.nextflow.io." + echo_yellow "NOTE: Nextflow is trying to use the Java VM defined by the following environment variables:\n JAVA_CMD: $JAVA_CMD\n NXF_OPTS: $NXF_OPTS\n" + exit 1 + fi + JAVA_VER=$(echo "$JAVA_VER" | awk '/version/ {gsub(/"/, "", $3); print $3}') + # check NF version + if [[ ! $NXF_VER =~ ([0-9]+)\.([0-9]+)\.([0-9].*) ]]; then + echo_red "Not a valid Nextflow version: $NXF_VER" + exit 1 + fi + major=${BASH_REMATCH[1]} + minor=${BASH_REMATCH[2]} + # legacy version - Java 7/8 only + if [ $major -eq 0 ] && [ $minor -lt 26 ]; then + version_check="^(1.7|1.8)" + version_message="Java 7 or 8" + else + version_check="^(1.8|9|10|11|12|13|14|15|16|17|18|19|20|21|22|23)" + version_message="Java 8 or later (up to 22)" + fi + if [[ ! $JAVA_VER =~ $version_check ]]; then + echo_red "ERROR: Cannot find Java or it's a wrong version -- please make sure that $version_message is installed" + if [[ "$NXF_JAVA_HOME" ]]; then + echo_yellow "NOTE: Nextflow is trying to use the Java VM defined by the following environment variables:\n JAVA_CMD: $JAVA_CMD\n NXF_JAVA_HOME: $NXF_JAVA_HOME\n" + else + echo_yellow "NOTE: Nextflow is trying to use the Java VM defined by the following environment variables:\n JAVA_CMD: $JAVA_CMD\n JAVA_HOME: $JAVA_HOME\n" + fi + exit 1 + fi + if [[ ! $JAVA_VER =~ ^(11|12|13|14|15|16|17|18|19|20|21|22|23) ]]; then + echo_yellow "NOTE: Nextflow is not tested with Java $JAVA_VER -- It's recommended the use of version 11 up to 23\n" + fi + mkdir -p "$(dirname "$JAVA_KEY")" + [[ -f $JAVA_VER ]] && echo $JAVA_VER > "$JAVA_KEY" +fi + +# Verify nextflow jar is available +if [ ! -f "$NXF_BIN" ]; then + [ -f "$NXF_PATH" ] && rm "$NXF_PATH" + mkdir -p "$NXF_PATH" || exit $? + tmpfile=$(make_temp) + get "$NXF_URL" "$tmpfile" "$NXF_BIN" + mv "$tmpfile" "$NXF_BIN" +fi + +COLUMNS=${COLUMNS:-`tty -s && tput cols 2>/dev/null || true`} +declare -a JAVA_OPTS=() +JAVA_OPTS+=(-Dfile.encoding=UTF-8 -Dcapsule.trampoline -Dcapsule.java.cmd="$JAVA_CMD" -Dcom.sun.security.enableAIAcaIssuers=true) +if [[ $cmd == console ]]; then bg=1; +else JAVA_OPTS+=(-Djava.awt.headless=true) +fi + +if [[ $NXF_LEGACY_LAUNCHER ]]; then + [[ "$JAVA_HOME" ]] && JAVA_OPTS+=(-Dcapsule.java.home="$JAVA_HOME") + [[ "$CAPSULE_LOG" ]] && JAVA_OPTS+=(-Dcapsule.log=$CAPSULE_LOG) + [[ "$CAPSULE_RESET" ]] && JAVA_OPTS+=(-Dcapsule.reset=true) +fi +[[ "$cmd" != "run" && "$cmd" != "node" ]] && JAVA_OPTS+=(-XX:+TieredCompilation -XX:TieredStopAtLevel=1) +[[ "$NXF_OPTS" ]] && JAVA_OPTS+=($NXF_OPTS) +[[ "$NXF_CLASSPATH" ]] && export NXF_CLASSPATH +[[ "$NXF_GRAB" ]] && export NXF_GRAB +[[ "$COLUMNS" ]] && export COLUMNS +[[ "$NXF_TEMP" ]] && JAVA_OPTS+=(-Djava.io.tmpdir="$NXF_TEMP") +[[ "${jvmopts[@]}" ]] && JAVA_OPTS+=("${jvmopts[@]}") +export JAVA_CMD +export CAPSULE_CACHE_DIR +export NXF_PLUGINS_DIR +export NXF_PLUGINS_MODE +export NXF_PLUGINS_DEFAULT +export NXF_PACK +export NXF_ENABLE_VIRTUAL_THREADS + +# lookup the a `md5` command +if hash md5sum 2>/dev/null; then MD5=md5sum; +elif hash gmd5sum 2>/dev/null; then MD5=gmd5sum; +elif hash md5 2>/dev/null; then MD5=md5; +else MD5='' +fi + +# when no md5 command is available fallback on default execution +if [ ! "$MD5" ] || [ "$CAPSULE_RESET" ]; then + launcher=($("$JAVA_CMD" "${JAVA_OPTS[@]}" -jar "$NXF_BIN")) + launch_nextflow + exit 1 +fi + +# creates a md5 unique for the given variables +env_md5() { +cat </dev/null; then + STR='' + for x in "${launcher[@]}"; do + [[ "$x" != "\"-Duser.dir=$PWD\"" ]] && [[ ! "$x" == *"-agentlib:jdwp"* ]] && STR+=$(printf '%q ' "$x") + done + printf "$STR">"$LAUNCH_FILE" + else + echo_yellow "Warning: Couldn't create cached classpath folder: $NXF_LAUNCHER -- Maybe NXF_HOME is not writable?" + fi + fi + +fi + +# check for latest version +check_latest +# finally run it +launch_nextflow diff --git a/.github/workflows/main.yml b/.github/workflows/main.yml new file mode 100644 index 0000000..b850f59 --- /dev/null +++ b/.github/workflows/main.yml @@ -0,0 +1,78 @@ +name: CI +on: [push, pull_request, workflow_dispatch] + +concurrency: + group: ${{ github.workflow }}-${{ github.event.pull_request.number || github.ref }} + cancel-in-progress: true + +jobs: + + test_rain: + + # Avoid running twice for a push + PR + if: github.event_name != 'pull_request' || github.event.pull_request.head.repo.full_name != github.event.pull_request.base.repo.full_name + + runs-on: ubuntu-24.04 + + services: + docker: + image: docker:dind + options: --privileged --shm-size=2g + volumes: + - /var/run/docker.sock:/var/run/docker.sock:ro + + steps: + + - name: Get the code + uses: actions/checkout@v3 + + # Install/cache OpenJDK + - name: Cache OpenJDK + id: cache-openjdk + uses: actions/cache@v3 + with: + path: /usr/lib/jvm/java-11-openjdk-amd64 + key: ${{ runner.os }}-openjdk-11 + restore-keys: | + ${{ runner.os }}-openjdk-11 + - name: Install OpenJDK + if: ${{ steps.cache-openjdk.outputs.cache-hit != 'true' }} + run: sudo apt-get install openjdk-11-jdk + + # Install/cache Nextflow + - name: Cache Nextflow + id: cache-nextflow + uses: actions/cache@v3 + with: + path: /usr/local/bin/nextflow + key: ${{ runner.os }}-nextflow + restore-keys: | + ${{ runner.os }}-nextflow + - name: Install Nextflow + if: ${{ steps.cache-nextflow.outputs.cache-hit != 'true' }} + run: cat .github/workflows/install_nextflow_v24.10.5.sh | bash && mv nextflow /usr/local/bin && chmod +x /usr/local/bin/nextflow + + # Build/cache containers + - name: Cache containers + id: cache-containers + uses: actions/cache@v3 + with: + key: ${{ runner.os }}-containers + path: /var/lib/docker/overlay2 + restore-keys: | + ${{ runner.os }}-containers + - name: Build Containers + if: ${{ steps.cache-containers.outputs.cache-hit != 'true'}} + run: bash build_all.sh + + # Check-out the repo under $GITHUB_WORKSPACE so that the job can access it + - uses: actions/checkout@v3 + + # Run test(s) + - name: test short single + run: nextflow run rain.nf -profile docker,test + + - name: test short paired + run: nextflow run rain.nf -profile docker,test2 + + diff --git a/config/ressources/base_aline.config b/config/ressources/base_aline.config new file mode 100644 index 0000000..761f7a6 --- /dev/null +++ b/config/ressources/base_aline.config @@ -0,0 +1,7 @@ +process { + cpus = 1 + time = '1h' + maxForks = 2 + shell = ['/bin/bash', '-euo', 'pipefail'] + stageOutMode = 'rsync' +} diff --git a/nextflow.config b/nextflow.config index ceb1746..cb20274 100644 --- a/nextflow.config +++ b/nextflow.config @@ -51,7 +51,7 @@ profiles { includeConfig "$baseDir/config/ressources/local.config" } test { - params.aline_profiles = "${baseDir}/config/ressources/custom_aline.config" + params.aline_profiles = "${baseDir}/config/ressources/base_aline.config" params.aligner = "STAR" params.reads = "${baseDir}/data/chr21/chr21_small_R1.fastq.gz " params.genome = "${baseDir}/data/chr21/chr21_small.fasta.gz" @@ -59,7 +59,7 @@ profiles { params.read_type = "short_single" } test2 { - params.aline_profiles = "${baseDir}/config/ressources/custom_aline.config" + params.aline_profiles = "${baseDir}/config/ressources/base_aline.config" params.aligner = "STAR" params.reads = "${baseDir}/data/chr21/" params.genome = "${baseDir}/data/chr21/chr21_small.fasta.gz"