diff --git a/methylcheck/QCreport.py b/methylcheck/QCreport.py new file mode 100644 index 0000000..633f866 --- /dev/null +++ b/methylcheck/QCreport.py @@ -0,0 +1,2 @@ +# -*- coding: utf-8 -*- + diff --git a/methylcheck/cli.py b/methylcheck/cli.py index 1510c11..f687bd3 100644 --- a/methylcheck/cli.py +++ b/methylcheck/cli.py @@ -47,9 +47,12 @@ def get_filename(array_name): if on_lambda: man_path = Path(MANIFEST_DIR_PATH_LAMBDA).expanduser() else: - man_path = Path(MANIFEST_DIR_PATH).expanduser() - man_filename = ARRAY_FILENAME[array_name] - man_filepath = Path(man_path, man_filename) + if array_name == "epicv2": + man_path = "../software/MethylationEPIC_v2.0" # location of the epicv2 manifest file + else: + man_path = Path(MANIFEST_DIR_PATH).expanduser() + man_filename = ARRAY_FILENAME[array_name] + man_filepath = Path(man_path, man_filename) return man_filepath # shape: should be wide, with more columns than rows. The larger dimension is the probe count. @@ -66,6 +69,8 @@ def get_filename(array_name): return 'epic+' if returns == 'name' else (ArrayType('epic+'), get_filename('epic+')) elif 860000 <= col_count <= 868000: # actual: 865860 return 'epic' if returns == 'name' else (ArrayType('epic'), get_filename('epic')) + elif 868001 <= col_count <= 1999999: # actual: 990k approx + return 'epic' if returns == 'name' else (ArrayType('epicv2'), get_filename('epicv2')) elif 200000 <= col_count <= 300000: # actual: 236685 C20, 262812 v2, 284762 V3-V4, MM285 293220 --- testing, changed max from 270k to 290k for v1.4.6 return 'mouse' if returns == 'name' else (ArrayType('mouse'), get_filename('mouse')) else: diff --git a/methylcheck/data_files/EPICv2_control.npy b/methylcheck/data_files/EPICv2_control.npy new file mode 100644 index 0000000..007ee1d Binary files /dev/null and b/methylcheck/data_files/EPICv2_control.npy differ diff --git a/methylcheck/data_files/EPICv2_sex.npy b/methylcheck/data_files/EPICv2_sex.npy new file mode 100644 index 0000000..b4f0d25 Binary files /dev/null and b/methylcheck/data_files/EPICv2_sex.npy differ diff --git a/methylcheck/data_files/epicv2_extC.csv b/methylcheck/data_files/epicv2_extC.csv new file mode 100644 index 0000000..ef72ffc --- /dev/null +++ b/methylcheck/data_files/epicv2_extC.csv @@ -0,0 +1,16186 @@ +CpG_chrm,x +chr1,cg00001583_BC11 +chr17,cg00002116_BC11 +chr17,cg00002809_BC11 +chr1,cg00002837_BC11 +chr1,cg00008647_BC11 +chr7,cg00010207_BC11 +chrX,cg00011200_BC11 +chr8,cg00011247_BC11 +chr17,cg00013569_BC11 +chr17,cg00014986_BC11 +chr2,cg00017826_BC11 +chr16,cg00018828_BC11 +chr8,cg00019576_BC11 +chr14,cg00019654_TC11 +chr12,cg00026119_BC11 +chr18,cg00027037_TC11 +chr1,cg00027570_BC11 +chr6,cg00028881_TC11 +chr1,cg00030466_BC11 +chr1,cg00033127_BC11 +chr2,cg00035316_BC11 +chr13,cg00035636_BC11 +chr16,cg00037223_TC11 +chr1,cg00037477_BC11 +chr2,cg00039627_TC11 +chr2,cg00039794_BC11 +chr17,cg00039801_BC11 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+chr9,nv-GRCh38-chr9-77797577-77797577-C-A_BC11 +chr9,rs1040870_BC11 +chr10,rs10796216_BC11 +chr4,rs13369115_BC11 +chr8,rs6991394_BC11 diff --git a/methylcheck/data_files/epicv2_extT.csv b/methylcheck/data_files/epicv2_extT.csv new file mode 100644 index 0000000..0b3bf1e --- /dev/null +++ b/methylcheck/data_files/epicv2_extT.csv @@ -0,0 +1,16186 @@ +CpG_chrm,Probe_ID,x +chr1,cg00001583_BC11,cg00001583_BC11 +chr17,cg00002116_BC11,cg00002116_BC11 +chr17,cg00002809_BC11,cg00002809_BC11 +chr1,cg00002837_BC11,cg00002837_BC11 +chr1,cg00008647_BC11,cg00008647_BC11 +chr7,cg00010207_BC11,cg00010207_BC11 +chrX,cg00011200_BC11,cg00011200_BC11 +chr8,cg00011247_BC11,cg00011247_BC11 +chr17,cg00013569_BC11,cg00013569_BC11 +chr17,cg00014986_BC11,cg00014986_BC11 +chr2,cg00017826_BC11,cg00017826_BC11 +chr16,cg00018828_BC11,cg00018828_BC11 +chr8,cg00019576_BC11,cg00019576_BC11 +chr14,cg00019654_TC11,cg00019654_TC11 +chr12,cg00026119_BC11,cg00026119_BC11 +chr18,cg00027037_TC11,cg00027037_TC11 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+chr5,nv-GRCh38-chr5-68295257-68295257-G-A_BC11,nv-GRCh38-chr5-68295257-68295257-G-A_BC11 +chr5,nv-GRCh38-chr5-68295300-68295300-G-C_BC11,nv-GRCh38-chr5-68295300-68295300-G-C_BC11 +chr5,nv-GRCh38-chr5-68295300-68295300-G-T_BC11,nv-GRCh38-chr5-68295300-68295300-G-T_BC11 +chr7,nv-GRCh38-chr7-140753355-140753355-C-A_BC11,nv-GRCh38-chr7-140753355-140753355-C-A_BC11 +chr7,nv-GRCh38-chr7-140781603-140781603-C-T_BO11,nv-GRCh38-chr7-140781603-140781603-C-T_BO11 +chr7,nv-GRCh38-chr7-140781605-140781605-A-C_TO11,nv-GRCh38-chr7-140781605-140781605-A-C_TO11 +chr21,nv-GRCh38-chr7-152265113-152265113-C-A_BC11,nv-GRCh38-chr7-152265113-152265113-C-A_BC11 +chr7,nv-GRCh38-chr7-152265113-152265113-C-T_TC11,nv-GRCh38-chr7-152265113-152265113-C-T_TC11 +chr7,nv-GRCh38-chr7-55154128-55154128-G-A_BC11,nv-GRCh38-chr7-55154128-55154128-G-A_BC11 +chr7,nv-GRCh38-chr7-55171177-55171177-G-A_BC11,nv-GRCh38-chr7-55171177-55171177-G-A_BC11 +chr7,nv-GRCh38-chr7-55171177-55171177-G-T_TC11,nv-GRCh38-chr7-55171177-55171177-G-T_TC11 +chr9,nv-GRCh38-chr9-77797577-77797577-C-A_BC11,nv-GRCh38-chr9-77797577-77797577-C-A_BC11 +chr9,rs1040870_BC11,rs1040870_BC11 +chr10,rs10796216_BC11,rs10796216_BC11 +chr4,rs13369115_BC11,rs13369115_BC11 +chr8,rs6991394_BC11,rs6991394_BC11 diff --git a/methylcheck/filters.py b/methylcheck/filters.py new file mode 100644 index 0000000..cbdcd5b --- /dev/null +++ b/methylcheck/filters.py @@ -0,0 +1,304 @@ +# -*- coding: utf-8 -*- +import pkg_resources +import numpy as np +import pandas as pd + + +def _import_probe_filter_list(array): + """Function to identify array type and import the + appropriate probe exclusion dataframe""" + + if array == 'IlluminaHumanMethylation450k': + path = 'data_files/450k_polymorphic_crossRxtve_probes.csv.gz' + filepath = pkg_resources.resource_filename(__name__, path) + filter_options = pd.read_csv(filepath) + elif array == 'IlluminaHumanMethylationEPIC': + path = 'data_files/EPIC_polymorphic_crossRxtve_probes.csv.gz' + filepath = pkg_resources.resource_filename(__name__, path) + filter_options = pd.read_csv(filepath) + else: + raise ValueError( + "Did not recognize array type. Please specify 'IlluminaHumanMethylation450k' or 'IlluminaHumanMethylationEPIC'.") + return + return filter_options + + +def _import_probe_exclusion_list(array, type): + """Returns a list of probe_ids based on type of array and type of probes to be excluded (sex or control). + + |array | sex_linked | controls| + |------|------------|---------| + |EPIC+ | 20137 | 755 | + |EPIC | 19627 | 695 | + |450k | 11648 | 916 | + |27k | | | + + Parameters + ---------- + array -- [EPIC, EPIC+, 450k, 27k] or ['IlluminaHumanMethylationEPIC', 'IlluminaHumanMethylationEPIC+', 'IlluminaHumanMethylation450k'] + type -- [sex, control]""" + + if array in ('IlluminaHumanMethylation450k','450k'): + path = 'data_files/450k_{0}.npy'.format(type) + elif array in ('IlluminaHumanMethylationEPICv2','EPICv2','epicv2'): + filename = 'data_files/EPICv2_{0}.npy'.format(_type) + elif array in ('IlluminaHumanMethylationEPIC','EPIC'): + path = 'data_files/EPIC_{0}.npy'.format(type) + elif array in ('IlluminaHumanMethylationEPIC+','EPIC+'): + path = 'data_files/EPIC+_{0}.npy'.format(type) + elif array =='27k': + raise NotImplementedError("We can't read 27k arrays yet.") + else: + raise ValueError("""Did not recognize array type. Please specify one of + 'IlluminaHumanMethylation450k', 'IlluminaHumanMethylationEPIC', '450k', 'EPIC', 'EPIC+', '27k'.""") + filepath = pkg_resources.resource_filename(__name__, path) + probe_list = np.load(filepath, allow_pickle=True) + return probe_list + + +def exclude_sex_control_probes(df, array, no_sex=True, no_control=True, verbose=False): + """Function to exclude probes from an array, and return a filtered array. + + Parameters + ---------- + df: dataframe of beta values or m-values. + array: type of array used. + {'27k', '450k', 'EPIC'} or {'IlluminaHumanMethylation27k','IlluminaHumanMethylation450k','IlluminaHumanMethylationEPIC',} + + Optional Arguments + ------------------ + `no_sex`: bool + (default True) + if True, will remove all probes that target X and Y chromosome locations, + as they are sex specific -- and lead to multiple clusters when trying to detect and remove outliers (noisy data). + + `no_control`: bool + (default True) + if True, removes Illumina's internal control probes. + + `verbose`: bool + (default False) + reports out on number of probes removed. + + Returns + ------- + a dataframe with samples removed.""" + translate = { + 'IlluminaHumanMethylationEPIC':'EPIC', + 'IlluminaHumanMethylation450k':'450k', + 'IlluminaHumanMethylation27k':'27k', + 'EPICv2': 'epicv2', + 'IlluminaHumanMethylationEPICv2': 'epicv2', + } + if array in translate: + array = translate[array] + exclude_sex = _import_probe_exclusion_list(array, 'sex') if no_sex == True else [] + exclude_control = _import_probe_exclusion_list(array, 'control') if no_control == True else [] + exclude = list(exclude_sex)+list(exclude_control) + + # first check shape of df; probes are in the longer axis. + FLIPPED = False + if len(df.columns) > len(df.index): + df = df.transpose() + FLIPPED = True # use this to reverse at end, so returned in original orientation. + + # next, actually remove probes from all samples matching these lists. + filtered = df.drop(exclude, errors='ignore') + + # errors = ignore: if a probe is missing from df, or present multiple times, drop what you can. + if verbose == True: + print("""Array {4}: Removed {0} sex linked probes and {1} internal control probes \ +from {2} samples. {3} probes remaining.""".format( + len(exclude_sex), + len(exclude_control), + len(filtered.columns), + len(filtered), + array + )) + discrepancy = (len(exclude_sex) + len(exclude_control)) - (len(df) - len(filtered)) + if discrepancy != 0: + print("Discrepancy between number of probes to exclude ({0}) and number actually removed ({1}): {2}".format( + len(exclude_sex) + len(exclude_control), + len(df) - len(filtered), + discrepancy + )) + if len(exclude_control) == discrepancy: + print("It appears that your sample had no control probes, or that the control probe names didn't match the manifest ({0}).".format(array)) + else: + print("This happens when probes are present multiple times in array, or the manifest doesn't match the array ({0}).".format(array)) + # reverse the FLIP + if FLIPPED: + filtered = filtered.transpose() + return filtered + +def exclude_probes(array, probe_list): + """ +How to: + use list_problem_probes to obtain a list of probes, then pass that in as a probe_list along with the dataframe of beta values (array) + +Resolves a problem whereby probe lists have basic names, but samples have additional meta data added. +Example: + +probe list + ['cg24168924', 'cg15886294', 'cg05943251', 'cg05579622', 'cg01797553', 'cg14885690', 'cg12490816', 'cg02631583', 'cg17361593', 'cg15000031', 'cg21515494', 'cg17219246', 'cg10838001', 'cg13913475', 'cg00492169', 'cg20352786', 'cg05932698', 'cg06736139', 'cg08333283', 'cg10010298', 'cg25984048', 'cg27287823', 'cg19269713', 'cg12456833', 'cg26161708', 'cg04984052', 'cg00033806', 'cg23255774', 'cg10717379', 'cg00880984', 'cg01818617', 'cg18563133', 'cg15895341', 'cg08155050', 'cg06820286', 'cg04325909', 'cg15094920', 'cg08037129', 'cg11161730', 'cg06044537', 'cg11936560', 'cg12404870', 'cg12670496', 'cg01473643', 'cg08605930', 'cg16553354', 'cg22175254', 'cg22966295', 'cg07346931', 'cg06234741'] +sample probe names + Index(['cg00000029_II_F_C_rep1_EPIC', 'cg00000103_II_F_C_rep1_EPIC', 'cg00000109_II_F_C_rep1_EPIC', 'cg00000155_II_F_C_rep1_EPIC', + 'cg00000158_II_F_C_rep1_EPIC', 'cg00000165_II_R_C_rep1_EPIC', 'cg00000221_II_R_C_rep1_EPIC', 'cg00000236_II_R_C_rep1_EPIC', + ... + 'ch.9.98957343R_II_R_O_rep1_EPIC', 'ch.9.98959675F_II_F_O_rep1_EPIC', 'ch.9.98989607R_II_R_O_rep1_EPIC', 'ch.9.991104F_II_F_O_rep1_EPIC'] + +This chops off anything after the first underscore, and compares with probe_list to see if percent match increases. +It then drops probes from array that match probe_list, at least partially. + +ADDED: checking whether array.index is string or int type. Regardless, this should work and not alter the original index.""" + + # 1 - check shape of array, to ensure probes are the index/values + ARRAY = array.index + if len(array.index) < len(array.columns): + ARRAY = array.columns + + pre_overlap = len(set(ARRAY) & set(probe_list)) + + # probe_list is always a list of strings. COERCING to strings here for matching. + array_probes = [str(probe).split('_')[0] for probe in list(ARRAY)] + post_overlap = len(set(array_probes) & set(probe_list)) + + if pre_overlap != post_overlap: + print("matching probes: {0} vs {1} after name fix, yielding {2} probes.".format(pre_overlap, post_overlap, len(array)-post_overlap)) + else: + print("Of {0} probes, {1} matched, yielding {2} probes after filtering.".format(len(array), post_overlap, len(array)-post_overlap)) + if post_overlap >= pre_overlap: + # match which probes to drop from array. + array_probes_lookup = {str(probe).split('_')[0]: probe for probe in list(ARRAY)} + # get a list of unmodified array_probe_names to drop, based on matching the overlapping list with the modified probe names. + exclude = [array_probes_lookup[probe] for probe in (set(array_probes) & set(probe_list))] + return array.drop(exclude, errors='ignore') + print("Nothing removed.") + return array + +def list_problem_probes(array, criteria=None, custom_list=None): + """Function to create a list of probes to exclude from downstream processes. + By default, all probes that have been noted in the literature to have + polymorphisms, cross-hybridization, repeat sequence elements and base color + changes are included in the DEFAULT exclusion list. + + - You can customize the exclusion list by passing in either publication shortnames or criteria into the function. + - you can combine pubs and reasons into the same list of exclusion criteria. + - if a publication doesn't match your array type, it will raise an error and tell you. + + Including any of these labels in pubs (publications) or criteria (described below) + will result in these probes NOT being included in the final exclusion list. + + User also has ability to add custom list of probes to include in final returned list. + + Parameters + ---------- + array: string + name for type of array used + 'IlluminaHumanMethylationEPIC', 'IlluminaHumanMethylation450k' + This shorthand names are also okay: + 'EPIC','EPIC+','450k','27k' + + criteria: list + List of the publications to use when excluding probes. + If the array is 450K the publications may include: + 'Chen2013' + 'Price2013' + 'Zhou2016' + 'Naeem2014' + 'DacaRoszak2015' + If the array is EPIC the publications may include: + 'Zhou2016' + 'McCarthey2016' + If no publication list is specified, probes from + all publications will be added to the exclusion list. + If more than one publication is specified, all probes + from all publications in the list will be added to the + exclusion list. + + criteria: lists + List of the criteria to use when excluding probes. + List may contain the following exculsion criteria: + 'Polymorphism' + 'CrossHybridizing' + 'BaseColorChange' + 'RepeatSequenceElements' + If no criteria list is specified, all critera will be + excluded. If more than one criteria is specified, + all probes meeting any of the listed criteria will be + added to the exclusion list. + + custom_list: list, default None + User-provided list of probes to be excluded. + These probe names have to match the probe names in your data exactly. + + Returns + ------- + probe_exclusion_list: list + List containing probe identifiers to be excluded""" + + translate = { + 'EPIC+': 'IlluminaHumanMethylationEPIC', + 'EPIC': 'IlluminaHumanMethylationEPIC', + '450k': 'IlluminaHumanMethylation450k', + '27k': 'IlluminaHumanMethylation27k', + } + + probe_pubs_translate = { + 'Price2013': 'Price_etal_2013', + 'Chen2013': 'Chen_etal_2013', + 'Naeem2014': 'Naeem_etal_2014', + 'DacaRoszak2015': 'Daca-Roszak_etal_2015', + 'McCartney2016': 'McCartney_etal_2016', + 'Zhou2016': 'Zhou_etal_2016', + } + if array in translate: + array = translate[array] + probe_dataframe = _import_probe_filter_list(array) + """ + I had no idea how this works, so I replaced it. MM July 2019. + -- syntax error when I ran it: these vars are not defined. + args = [Polymorphism, CrossHybridization, + BaseColorChange, RepeatSequenceElements, + Chen2013, Price2013, Zhou2016, Naeem2014, + DacaRoszak2015, McCartney2016]""" + if not custom_list: + custom_list = [] + if not criteria: + probe_exclusion_list = list(set(probe_dataframe['Probe'].values)) + probe_exclusion_list = list(set(probe_exclusion_list + custom_list)) + return probe_exclusion_list + else: + probe_exclusion_list = [] + + # rename criteria to match probe_dataframe names, or pass through. + criteria = [probe_pubs_translate.get(crit, crit) for crit in criteria] + + reasons = ['Polymorphism', 'CrossHybridization', 'BaseColorChange', 'RepeatSequenceElements'] + for reason in criteria: + if reason not in reasons: + continue + df = probe_dataframe[probe_dataframe['Reason'] == reason] + probe_exclusion_list = list( + set(df['Probe'].values)) + probe_exclusion_list + + epic_pubs = ['Zhou_etal_2016', 'McCartney_etal_2016'] + fourfifty_pubs = ['Chen_etal_2013', 'Price_etal_2013', 'Naeem_etal_2014', 'Daca-Roszak_etal_2015'] + for pub in criteria: + if pub not in (epic_pubs + fourfifty_pubs): + continue + + if pub in fourfifty_pubs and array == 'IlluminaHumanMethylationEPIC': + raise ValueError( + "Citation {0} does not exist for '{1}'.".format(pub, array)) + + if pub in epic_pubs and array == 'IlluminaHumanMethylation450k': + raise ValueError( + "Citation {0} does not exist for '{1}'.".format(pub, array)) + + df = probe_dataframe[probe_dataframe['ShortCitation'] == pub] + probe_exclusion_list = list( + set(df['Probe'].values)) + probe_exclusion_list + + probe_exclusion_list = list(set(probe_exclusion_list + custom_list)) + return probe_exclusion_list diff --git a/methylcheck/postprocessQC.py b/methylcheck/postprocessQC.py new file mode 100644 index 0000000..d76a51d --- /dev/null +++ b/methylcheck/postprocessQC.py @@ -0,0 +1,676 @@ +# -*- coding: utf-8 -*- +# built ins +import logging +import os +import datetime + +# package +import pandas as pd +import numpy as np +import seaborn as sns +import matplotlib.pyplot as plt +import matplotlib.lines as mlines +from sklearn.manifold import MDS +from tqdm import tqdm + +LOGGER = logging.getLogger(__name__) + + +def mean_beta_plot(df, verbose=False, save=False, silent=False): + """Returns a plot of the average beta values for all probes in a batch of samples. + + Input (df): + - a dataframe with probes in rows and sample_ids in columns. + - to get this formatted import, use `methylprep.consolidate_values_for_sheet()`, + as this will return a matrix of beta-values for a batch of samples (by default).""" + if df.shape[0] < df.shape[1]: + ## ensure probes in rows and samples in cols + if verbose: + print("Your data needed to be transposed (df = df.transpose()).") + LOGGER.info("Your data needed to be transposed (df = df.transpose()).") + df = df.copy().transpose() # don't overwrite the original + + data = df.copy(deep=True) + data['mean'] = data.mean(numeric_only=True, axis=1) + fig, ax = plt.subplots(figsize=(12, 9)) + sns.distplot(data['mean'], hist=False, rug=False, ax=ax, axlabel='beta') + plt.title('Mean Beta Plot') + plt.grid() + plt.xlabel('Mean Beta') + if save: + plt.savefig('mean_beta.png') + if not silent: + plt.show() + else: + plt.close(fig) + + +def beta_density_plot(df, verbose=False, save=False, silent=False): + """Returns a plot of beta values for each sample in a batch of samples as a separate line. + Y-axis values is the count (of what? intensity? normalized?). + X-axis values are beta values (0 to 1) for a single samples + + Input (df): + - a dataframe with probes in rows and sample_ids in columns. + - to get this formatted import, use `methylprep.consolidate_values_for_sheet()`, + as this will return a matrix of beta-values for a batch of samples (by default). + + Returns: + None""" + if df.shape[0] < df.shape[1]: + ## ensure probes in rows and samples in cols + if verbose: + print("Your data needed to be transposed (df = df.transpose()).") + LOGGER.info("Your data needed to be transposed (df = df.transpose()).") + df = df.copy().transpose() # don't overwrite the original + + fig, ax = plt.subplots(figsize=(12, 9)) + + for col in df.columns: + if col != 'Name': # probe name + if len(df.columns) <= 30: + sns.distplot( + df[col], hist=False, rug=False, + label=col, ax=ax, axlabel='beta') + else: + sns.distplot( + df[col], hist=False, rug=False, axlabel='beta') + + if len(df.columns) <= 30: + plt.legend(bbox_to_anchor=(1.05, 1), loc=2, borderaxespad=0.) + #else: + # ax.get_legend().set_visible(False) + # print('suppressing legend') + + plt.title('Beta Density Plot') + plt.grid() + plt.xlabel('Beta values') + if save: + plt.savefig('beta.png') + if not silent: + plt.show() + else: + plt.clf() + plt.cla() + plt.close() + + +def cumulative_sum_beta_distribution(df, cutoff=0.7, verbose=False, save=False, silent=False): + """ attempts to filter outlier samples based on the cumulative area under the curve + exceeding a reasonable value (cutoff). + + Inputs: + DataFrame -- wide format (probes in columns, samples in rows) + cutoff (default 0.7) + silent -- suppresses figure, so justs returns transformed data if False. + if save==True: saves figure to disk. + + Returns: + dataframe with subjects removed that exceed cutoff value.""" + # ensure probes in colums, samples in rows + if df.shape[1] < df.shape[0]: + df = df.copy().transpose() # don't overwrite the original + if verbose: + print("Your data needed to be transposed (df = df.transpose()).") + LOGGER.info("Your data needed to be transposed (df = df.transpose()).") + + good_samples = [] + outliers = [] + if not silent: + print("Calculating area under curve for each sample.") + fig, ax = plt.subplots(figsize=(12, 9)) + # first, check if probes aren't consistent, to avoid a crash here + df = drop_nan_probes(df, silent=silent, verbose=verbose) + + # if silent is True, tqdm will not show process bar. + for subject_num, (row, subject_id) in tqdm(enumerate(zip(df.values, + df.index)), disable=silent): + hist_vals = np.histogram(row, bins=10)[0] + hist_vals = hist_vals / np.sum(hist_vals) + cumulative_sum = np.cumsum(hist_vals) + if cumulative_sum[5] < cutoff: + good_samples.append(subject_num) + sns.distplot(row, hist=False, norm_hist=False) + else: + outliers.append(subject_id) # drop uses ids, not numbers. + + plt.title('Cumulative Sum Beta Distribution (filtered at {0})'.format(cutoff)) + plt.grid() + if len(df.columns) <= 30: + plt.legend(bbox_to_anchor=(1.05, 1), loc=2, borderaxespad=0.) + else: + ax.legend_ = None + if save: + plt.savefig('cum_beta.png') + if not silent: + plt.show() + plt.clf() + plt.cla() + plt.close() + return df.drop(outliers, axis=0) + + +def beta_mds_plot(df, filter_stdev=1.5, verbose=True, save=False, silent=False, multi_params={'draw_box':True}): + """ + 1 needs to read the manifest file for the array, or at least a list of probe names to exclude/include. + manifest_file = pd.read_csv('/Users/nrigby/GitHub/stp-prelim-analysis/working_data/CombinedManifestEPIC.manifest.CoreColumns.csv')[['IlmnID', 'CHR']] + probe_names_no_sex_probes = manifest_file.loc[manifest_file['CHR'].apply(lambda x: x not in ['X', 'Y', np.nan]), 'IlmnID'].values + probe_names_sex_probes = manifest_file.loc[manifest_file['CHR'].apply(lambda x: x in ['X', 'Y']), 'IlmnID'].values + + df_no_sex_probes = df[probe_names_no_sex_probes] + df_no_sex_probes.head() + + Arguments + --------- + df + dataframe of beta values for a batch of samples (rows are probes; cols are samples) + filter_stdev + a value (unit: standard deviations) between 0 and 3 (typically) that represents + the fraction of samples to include, based on the standard deviation of this batch of samples. + So using the default value of 1.5 means that all samples whose MDS-transformed beta sort_values + are within +/- 1.5 standard deviations of the average beta are retained in the data returned. + multi_params + is a dict, passed into this function from a multi-compare-MDS wrapper function, containing: + {return_plot_obj=True, + fig=None, + ax=None, + draw_box=False, + xy_lim=None, + color_num=0, + PSF=1.2 -- plot scale factor (margin beyond points to display)} + + Options + -------- + silent + if running from command line in an automated process, you can run in `silent` mode to suppress any user interaction. + In this case, whatever `filter_stdev` you assign is the final value, and a file will be processed with that param. + Silent also suppresses plots (images) from being generated. only files are returned. + + returns + ------- + returns a filtered dataframe. + if `return_plot_obj` is True, it returns the plot, for making overlays in methylize. + + requires + -------- + pandas, numpy, pyplot, sklearn.manifold.MDS """ + + # ensure "long format": probes in rows and samples in cols. This is how methylprep returns data. + if df.shape[1] < df.shape[0]: + ## methylcheck needs probes in rows and samples in cols. but MDS needs a wide matrix. + df = df.copy().transpose() # don't overwrite the original + if verbose: + print("Your data needed to be transposed (df = df.transpose()).") + LOGGER.info("Your data needed to be transposed (df = df.transpose()).") + if verbose == True: + print(df.shape) + df.head() + LOGGER.info('DataFrame has shape: {0}'.format(df.shape)) + print("Making sure that probes are in columns (the second number should be larger than the first).") + LOGGER.info("Making sure that probes are in columns (the second number should be larger than the first).") + # before running this, you'd typically exclude probes. + print("Starting MDS fit_transform. this may take a while.") + LOGGER.info("Starting MDS fit_transform. this may take a while.") + + #df = drop_nan_probes(df, silent=silent, verbose=verbose) + + # CHECK for missing probe values NaN + missing_probe_counts = df.isna().sum() + total_missing_probes = sum([i for i in missing_probe_counts])/len(df) # sum / columns + if sum([i for i in missing_probe_counts]) > 0: + df = df.dropna() + if verbose == True: + print("We found {0} probe(s) were missing values and removed them from MDS calculations.".format(total_missing_probes)) + if silent == False: + LOGGER.info("{0} probe(s) were missing values removed from MDS calculations.".format(total_missing_probes)) + + mds = MDS(n_jobs=-1, random_state=1, verbose=1) + #n_jobs=-1 means "use all processors" + mds_transformed = mds.fit_transform(df.values) + # pass in df.values (a np.array) instead of a dataframe, as it processes much faster. + # old range is used for plotting, with some margin on outside for chart readability + + # plot_scale_fator -- an empirical number to stretch the plot out and show clusters more easily. + if 'PSF' in multi_params: + PSF = multi_params.get('PSF') + else: + PSF = 2 + + if df.shape[0] < 40: + DOTSIZE = 16 + elif 40 < df.shape[0] < 60: + DOTSIZE = 14 + elif 40 < df.shape[0] < 60: + DOTSIZE = 12 + elif 60 < df.shape[0] < 80: + DOTSIZE = 10 + elif 80 < df.shape[0] < 100: + DOTSIZE = 8 + elif 100 < df.shape[0] < 300: + DOTSIZE = 7 + else: + DOTSIZE = 5 + old_X_range = [min(mds_transformed[:, 0]), max(mds_transformed[:, 0])] + old_Y_range = [min(mds_transformed[:, 1]), max(mds_transformed[:, 1])] + #old_X_range = [old_X_range[0] - PSF*old_X_range[0], old_X_range[1] + PSF*old_X_range[1]] + #old_Y_range = [old_Y_range[0] - PSF*old_Y_range[0], old_Y_range[1] + PSF*old_Y_range[1]] + x_std, y_std = np.std(mds_transformed,axis=0) + x_avg, y_avg = np.mean(mds_transformed,axis=0) + + adj = filter_stdev #(1.5) + + if verbose == True: + print("""You can now remove outliers based on their transformed beta values + falling outside a range, defined by the sample standard deviation.""") + + while True: + df_indexes_to_retain = [] + df_indexes_to_exclude = [] + # xy_lim is a manual override preset boundary cutoff range for MDS filtering. + if multi_params.get('xy_lim'): + xy_lim = multi_params['xy_lim'] + if type(xy_lim) in (list,tuple): # ((xmin, xmax), (ymin, ymax)) + minX = xy_lim[0][0] + maxX = xy_lim[0][1] + minY = xy_lim[1][0] + maxY = xy_lim[1][1] + else: + raise ValueError ("xy_lim in multi_params must be a list or tuple") + else: + minX = round(x_avg - adj*x_std) + maxX = round(x_avg + adj*x_std) + minY = round(y_avg - adj*y_std) + maxY = round(y_avg + adj*y_std) + + if verbose == True: + print('Your acceptable value range: x=({0} to {1}), y=({2} to {3}).'.format( + minX, maxX, + minY, maxY + )) + # md2 are the dots that fall inside the cutoff. + md2 = [] + for idx,row in enumerate(mds_transformed): + if minX <= row[0] <= maxX and minY <= row[1] <= maxY: + md2.append(row) + df_indexes_to_retain.append(idx) + else: + df_indexes_to_exclude.append(idx) + #pandas style: mds2 = mds_transformed[mds_transformed[:, 0] == class_number[:, :2] + + # this is a np array, not a df. Remove all dots that are retained from the "exluded" data set (mds_transformed)) + mds_transformed = np.delete(mds_transformed, [df_indexes_to_retain], axis=0) + md2 = np.array(md2) + + color_num = multi_params.get('color_num',0) + # ADD TO EXISTING FIG... up to 24 colors (repeated 3x for 72 total) + COLORSET = dict(enumerate(['xkcd:blue', 'xkcd:green', 'xkcd:coral', 'xkcd:lightblue', 'xkcd:magenta', 'xkcd:goldenrod', 'xkcd:plum', 'xkcd:beige', + 'xkcd:orange', 'xkcd:orchid', 'xkcd:silver', 'xkcd:purple', 'xkcd:pink', 'xkcd:teal', 'xkcd:tomato', 'xkcd:yellow', + 'xkcd:olive', 'xkcd:lavender', 'xkcd:indigo', 'xkcd:black', 'xkcd:azure', 'xkcd:brown', 'xkcd:aquamarine', 'xkcd:darkblue', + + 'xkcd:blue', 'xkcd:green', 'xkcd:coral', 'xkcd:lightblue', 'xkcd:magenta', 'xkcd:goldenrod', 'xkcd:plum', 'xkcd:beige', + 'xkcd:orange', 'xkcd:orchid', 'xkcd:silver', 'xkcd:purple', 'xkcd:pink', 'xkcd:teal', 'xkcd:tomato', 'xkcd:yellow', + 'xkcd:olive', 'xkcd:lavender', 'xkcd:indigo', 'xkcd:black', 'xkcd:azure', 'xkcd:brown', 'xkcd:aquamarine', 'xkcd:darkblue', + + 'xkcd:blue', 'xkcd:green', 'xkcd:coral', 'xkcd:lightblue', 'xkcd:magenta', 'xkcd:goldenrod', 'xkcd:plum', 'xkcd:beige', + 'xkcd:orange', 'xkcd:orchid', 'xkcd:silver', 'xkcd:purple', 'xkcd:pink', 'xkcd:teal', 'xkcd:tomato', 'xkcd:yellow', + 'xkcd:olive', 'xkcd:lavender', 'xkcd:indigo', 'xkcd:black', 'xkcd:azure', 'xkcd:brown', 'xkcd:aquamarine', 'xkcd:darkblue'])) + + if multi_params.get('fig') == None: + fig = plt.figure(figsize=(12, 9)) + plt.title('MDS Plot of betas from methylation data') + plt.grid() # adds fig.axes implied + else: + fig = multi_params.get('fig') + + if multi_params.get('ax') == None and fig.axes != []: + if verbose: + print('axes', multi_params.get('ax'), fig.axes, 'assigned to ax.') + ax = fig.axes[0] # appears to get implicitly created when plot.grid() runs + elif multi_params.get('ax') == None: + # passing the 'ax' (fig.axes[0]) object back in will avoid the plotlib warning. + ax = fig.add_subplot(1,1,1) + else: + ax = multi_params.get('ax') + + ax.scatter(md2[:, 0], md2[:, 1], s=DOTSIZE, c=COLORSET.get(color_num,'black')) # RETAINED + ax.scatter(mds_transformed[:, 0], mds_transformed[:, 1], s=DOTSIZE, c='xkcd:ivory', edgecolor='black', linewidth='0.2',) # EXCLUDED + + x_range_min = PSF*old_X_range[0] if PSF*old_X_range[0] < minX else PSF*minX + x_range_max = PSF*old_X_range[1] if PSF*old_X_range[1] > maxX else PSF*maxX + y_range_min = PSF*old_Y_range[0] if PSF*old_Y_range[0] < minY else PSF*minY + y_range_max = PSF*old_Y_range[1] if PSF*old_Y_range[1] > maxY else PSF*maxY + ax.set_xlim([x_range_min, x_range_max]) + ax.set_ylim([y_range_min, y_range_max]) + #print(int(x_range_min), int(x_range_max), int(y_range_min), int(y_range_max)) + + if multi_params.get('draw_box') == True: + ax.vlines([minX, maxX], minY, maxY, color=COLORSET.get(color_num,'red'), linestyle=':') + ax.hlines([minY, maxY], minX, maxX, color=COLORSET.get(color_num,'red'), linestyle=':') + + if multi_params.get('return_plot_obj') == True: + return fig, ax, df_indexes_to_retain + + if silent == True: + # take the original dataframe (df) and remove samples that are outside the sample thresholds, returning a new dataframe + df.drop(df.index[df_indexes_to_exclude], inplace=True) + image_name = df.index.name or 'beta_mds_n={0}_p={1}'.format(len(df.index), len(df.columns)) # np.size(df,0), np.size(md2,1) + outfile = '{0}_s={1}_{2}.png'.format(image_name, filter_stdev, datetime.date.today()) + plt.savefig(outfile) + LOGGER.info("Saved {0}".format(outfile)) + plt.close(fig) + # returning DataFrame in original structure: rows are probes; cols are samples. + return df # may need to transpose this first. + else: + plt.show() + plt.close(fig) + + ########## BEGIN INTERACTIVE MODE ############# + print("Original samples {0} vs filtered {1}".format(mds_transformed.shape, md2.shape)) + print('Your scale factor was: {0}'.format(adj)) + adj = input("Enter new scale factor, to accept and save:") + if adj == '': + break + try: + adj = float(adj) + except ValueError: + print("Not a valid number. Type a number with a decimal value, or Press to quit.") + continue + + prev_df = len(df) + if save: + # save file. return dataframe. + fig = plt.figure(figsize=(12, 9)) + plt.title('MDS Plot of betas from methylation data') + plt.grid() # adds fig.axes implied + ax = fig.add_subplot(1,1,1) + ax.scatter(md2[:, 0], md2[:, 1], s=DOTSIZE, c=COLORSET.get(color_num,'black')) # RETAINED + ax.scatter(mds_transformed[:, 0], mds_transformed[:, 1], s=DOTSIZE, c='xkcd:ivory', edgecolor='black', linewidth='0.2',) # EXCLUDED + ax.set_xlim(old_X_range) + ax.set_ylim(old_Y_range) + # take the original dataframe (df) and remove samples that are outside the sample thresholds, returning a new dataframe + df_out = df.drop(df.index[df_indexes_to_exclude]) # inplace=True will modify the original DF outside this function. + + # UNRESOLVED BUG. + # was getting 1069 samples back from this; expected 1076. discrepancy is because + # pre_df_excl = len(df.index[df_indexes_to_exclude]) + # unique_df_excl = len(set(df.index[df_indexes_to_exclude])) + # print(pre_df_excl, unique_df_excl) + + image_name = df.index.name or 'beta_mds_n={0}_p={1}'.format(len(df.index), len(df.columns)) # np.size(df,0), np.size(md2,1) + outfile = '{0}_s={1}_{2}.png'.format(image_name, filter_stdev, datetime.date.today()) + plt.savefig(outfile) + plt.close(fig) # avoids displaying plot again in jupyter. + if verbose: + print("Saved {0}".format(outfile)) + LOGGER.info("Saved {0}".format(outfile)) + else: + df_out = df + # returning DataFrame in original structure: rows are probes; cols are samples. + return df_out #, df_indexes_to_exclude # may need to transpose this first. + + +def mean_beta_compare(df1, df2, save=False, verbose=False, silent=False): + """Use this function to compare two dataframes, pre-vs-post filtering and removal of outliers. + + silent: suppresses figure, so no output unless save==True too.""" + if df1.shape[0] < df1.shape[1]: + ## ensure probes in rows and samples in cols + if verbose: + print("Your first data set needed to be transposed (df = df.transpose()).") + LOGGER.info("Your data needed to be transposed (df = df.transpose()).") + df1 = df1.copy().transpose() # don't overwrite the original + if df2.shape[0] < df2.shape[1]: + ## ensure probes in rows and samples in cols + if verbose: + print("Your second data set needed to be transposed (df = df.transpose()).") + LOGGER.info("Your data needed to be transposed (df = df.transpose()).") + df2 = df2.copy().transpose() # don't overwrite the original + + data1 = df1.copy(deep=True) + data1['mean'] = data1.mean(numeric_only=True, axis=1) + data2 = df2.copy(deep=True) + data2['mean'] = data2.mean(numeric_only=True, axis=1) + + fig, ax = plt.subplots(figsize=(12, 9)) + line1 = sns.distplot(data1['mean'], hist=False, rug=False, ax=ax, axlabel='beta', color='xkcd:blue') + line2 = sns.distplot(data2['mean'], hist=False, rug=False, color='xkcd:green') + plt.title('Mean Beta Plot (Compare pre (blue) vs post (green) filtering)') + plt.grid() + plt.xlabel('Mean Beta') + #plt.legend([line1, line2], ['pre','post'], bbox_to_anchor=(1.05, 1), loc=2, borderaxespad=0.) + if save: + plt.savefig('mean_beta_compare.png') + if not silent: + plt.show() + else: + plt.close('all') + + +def combine_mds(*args, **kwargs): + """ + combine (or segment) datasets how it works + ------------------------------------------ + Use this function on multiple dataframes to combine datasets, or to visualize + parts of the same dataset in separate colors. It is a wrapper of `methylcheck.beta_mds_plot()` and applies + multidimensional scaling to cluster similar samples based on patterns in probe values, as well as identify + possible outlier samples (and exclude them). + + - combine datasets, + - run MDS, + - see how each dataset (or subset) overlaps with the others on a plot, + - exclude outlier samples based on a composite cutoff box (the average bounds of the component data sets) + - calculate the percent of data excluded from the group + + inputs + ------ + - *args: pass in any number of pandas dataframes, and it will combine them into one mds plot. + - alternatively, you may pass in a list of filepaths as strings, and it will attempt to load these files as pickles. + but they must be pickles of pandas dataframes + + optional keyword arguments + -------------------------- + - silent: (default False) + (automated processing mode) + if True, suppresses most information and avoids prompting user for anything. + silent mode processes data but doesn't show the plot. + - save: (default False) + if True, saves the plot png to disk. + - verbose: (default False) + if True, prints extra debug information to screen or logger. + + analysis parameters + ------------------- + - filter_stdev (how broadly should you retain samples? units are standard deviations, defaulting to 1.5 STDEV.) + if you increase this number, fewer outlier samples will be removed. + + returns + ------ + - TODO: one dataframe of the retained samples, cutoff box is avg of datasets + ~~nothing returned (currently)~~ + - TODO: each dataset's results as a transformed file + - default: list of samples retained or excluded + - option: a list of pyplot subplot objects + """ + + # check if already dataframes, or are they strings? + list_of_dfs = list(args) + if any([not isinstance(item, pd.DataFrame) for item in list_of_dfs]): + if set([type(item) for item in list_of_dfs]) == {str}: + try: + list_of_dfs = _load_data(list_of_dfs) + except Exception as e: + raise FileNotFoundError ("Either your files don't exist, or they are not pickled: {0}".format(e)) + # kwargs + save = kwargs.get('save', False) + silent = kwargs.get('silent', False) + verbose = kwargs.get('verbose', True) + filter_stdev = kwargs.get('filter_stdev', 1.5) + output_format = kwargs.get('output') + PRINT = print if verbose else _noprint + + # data to combine + dfs = pd.DataFrame() + # OPTIONAL OUTPUT: TRACK each source df's samples for plot color coding prior to merging + # i.e. was this sample included or excluded at end? + # maybe use a simple class here to track the data as it is being manipulated? + sample_source = {} + frame_transposed = {} + # subplots: possibly useful - list of subplot objects within the figure, and their metadata. + subplots = [] + # track x/y ranges to adjust plot area later + xy_lims = [] + + # PROCESS MDS + fig = None + ax = None + for idx, df in enumerate(list_of_dfs): + transposed = False + if df.shape[1] > df.shape[0]: # put probes in rows + df = df.transpose() + transposed = True + for sample in df.columns: + if sample in sample_source: + print("WARNING: sample names are not unique across data sets") + sample_source[sample] = idx + frame_transposed[idx] = transposed + + # first, PLOT separate MDS, each with its own box. + try: + fig,ax,df_indexes_to_retain = beta_mds_plot(df, filter_stdev=filter_stdev, save=save, verbose=verbose, silent=silent, + multi_params={'return_plot_obj':True, + 'fig':fig, + 'ax':ax, + 'color_num':idx, + 'draw_box':True, + 'PSF':1.2, + }) + subplots.append(fig) + xy_lims.append( (fig.axes[0].get_xlim(), fig.axes[0].get_ylim()) ) # (x_range, y_range) + except Exception as e: + PRINT(e) + + #set max range + x_range_min = min(item[0][0] for item in xy_lims) + x_range_max = max(item[0][1] for item in xy_lims) + y_range_min = min(item[1][0] for item in xy_lims) + y_range_max = max(item[1][1] for item in xy_lims) + fig.axes[0].set_xlim([x_range_min, x_range_max]) + fig.axes[0].set_ylim([y_range_min, y_range_max]) + #PRINT('full chart range', int(x_range_min), int(x_range_max), int(y_range_min), int(y_range_max)) + if not silent: + plt.show() + plt.close('all') + + # PART 2 - calculate the average MDS QC score + #1 run the loop. calc avg MDS boundary box for group. + #2 rerun the loop with this as the specified boundary. + #3 calculate percent retained per dataset + #4 overall QC score is avg percent retained per dataset. + avg_x_range_min = int(sum(item[0][0] for item in xy_lims)/len(xy_lims)) + avg_x_range_max = int(sum(item[0][1] for item in xy_lims)/len(xy_lims)) + avg_y_range_min = int(sum(item[1][0] for item in xy_lims)/len(xy_lims)) + avg_y_range_max = int(sum(item[1][1] for item in xy_lims)/len(xy_lims)) + xy_lim = ((avg_x_range_min, avg_x_range_max), (avg_y_range_min, avg_y_range_max)) + PRINT('Average MDS window coordinate range: (x: {0}, y:{1}'.format(xy_lim[0], xy_lim[1])) + fig = None + ax = None + transformed_dfs = [] + for idx, df in enumerate(list_of_dfs): + if df.shape[1] > df.shape[0]: # put probes in rows + df = df.transpose() + fig,ax,df_indexes_to_retain = beta_mds_plot(df, + filter_stdev=filter_stdev, save=save, verbose=verbose, silent=silent, + multi_params={ + 'return_plot_obj':True, + 'fig':fig, + 'ax':ax, + 'color_num':idx, + 'draw_box':True, + 'xy_lim':xy_lim, + 'PSF':1.2, + }) + try: + if frame_transposed[idx]: + # this data (df) was transposed from file-orientation, so samples are in rows now; probes in columns + df_transformed = df.iloc[df_indexes_to_retain, :] # samples in rows + print(df.shape, df_transformed.shape) + else: + # iloc: first list is row index; 2nd is column index + df_transformed = df.iloc[:, df_indexes_to_retain] # samples in columns + print(df.shape, df_transformed.shape) + transformed_dfs.append(df_transformed) + except: + import pdb;pdb.set_trace() + fig.axes[0].set_xlim([x_range_min, x_range_max]) + fig.axes[0].set_ylim([y_range_min, y_range_max]) + + # https://stackoverflow.com/questions/32213889/get-positions-of-points-in-pathcollection-created-by-scatter + #print(len(fig.axes[0].collections)) # collections has data in every 4th list item. + # items in the collections after the main data set are the excluded points + all_coords = [] + retained = [] + excluded = [] + retained_sample_dfs = [] + for i in range(0, 4*len(list_of_dfs), 1): + DD = fig.axes[0].collections[i] + DD.set_offset_position('data') + #print(DD.get_offsets()) + all_coords.extend(DD.get_offsets().tolist()) + #print(i, len(all_coords)) + if i % 4 == 0: # 0, 4, 8 -- this is the first data set applied to plot. (x,y plot coords) + retained.extend( DD.get_offsets().tolist() ) + # go from plot sample x,y to idx of samples in original dfs. + if i % 4 == 1: # 1, 5, 9, etc -- this is the second data set applied to plot. + excluded.extend( DD.get_offsets().tolist() ) + if verbose: + PRINT('{0} % retained overall ({1} out of {2} samples)'.format( + round(100*len(retained) / (len(retained) + len(excluded))), + len(retained), len(retained) + len(excluded) )) + if not silent: + plt.show() + plt.close('all') + + # TODO: output_format + return transformed_dfs + + +def _load_data(filepaths): + dfs = [] + for ff in filepaths: + df = pd.read_pickle(ff) + dfs.append(df) + return dfs + + +def _noprint(*messages): + """ a helper function to suppress print() if not verbose mode. """ + pass + + +def drop_nan_probes(df, silent=False, verbose=False): + """ accounts for df shape (probes in rows or cols) so dropna() will work. + + the method used inside MDS may be faster, but doesn't tell you which probes were dropped.""" + ### histogram can't have NAN values -- so need to exclude before running, or warn user. + # from https://dzone.com/articles/pandas-find-rows-where-columnfield-is-null -- returns a slimmer df of col/rows with NAN. + dfnan = df[df.isnull().any(axis=1)][df.columns[df.isnull().any()]] + if len(dfnan) > 0 and df.shape[0] > df.shape[1]: # a list of probe names that contain nan. + #probes in rows + pre_shape = df.shape + df = df.dropna() + note = "(probes,samples)" + if not silent: + LOGGER.info(f"dropping probe(s) that are missing a value (for this calculation): {dfnan}") + LOGGER.info(f"retained {df.shape} {note} from the original {pre_shape} {note}.") + if verbose: + print("We found {0} probe(s) were missing values and removed them from calculations.".format(len(dfnan))) + elif len(dfnan.columns) > 0 and df.shape[1] > df.shape[0]: + pre_shape = df.shape + df = df.dropna(axis='columns') + note = "(samples,probes)" + if not silent: + LOGGER.info(f"dropping probe(s) that are missing a value (for this calculation): {dfnan.columns}") + LOGGER.info(f"retained {df.shape} {note} from the original {pre_shape} {note}.") + if verbose: + print("We found {0} probe(s) were missing values and removed them from calculations.".format(len(dfnan.columns))) + return df diff --git a/methylcheck/predict/sex.py b/methylcheck/predict/sex.py index 55a8b28..8606a2d 100644 --- a/methylcheck/predict/sex.py +++ b/methylcheck/predict/sex.py @@ -71,7 +71,7 @@ def get_sex(data_source, array_type=None, verbose=False, plot=False, save=False, This feature won't work if a containers object or tuple of dataframes is passed in, instead of a path. Note: ~90% of Y probes should fail if the sample is female. That chromosome is missing.""" - allowed_array_types = {'27k','450k','epic','epic+','mouse'} + allowed_array_types = {'27k','450k','epic','epic+', 'epicv2', 'mouse'} try: from methylprep.files import Manifest @@ -129,11 +129,15 @@ def get_sex(data_source, array_type=None, verbose=False, plot=False, save=False, array_type = ArrayType(array_type) else: raise ValueError(f"Your array_type must be one of these: {allowed_array_types} or None.") - + print("Array type:", array_type) + if verbose: LOGGER.debug(array_type) LOGGER.setLevel(logging.WARNING) - manifest = Manifest(array_type, on_lambda=on_lambda, verbose=verbose)._Manifest__data_frame # 'custom', '27k', '450k', 'epic', 'epic+' + if str(array_type) == 'epicv2': + manifest = Manifest(array_type, filepath_or_buffer='../software/MethylationEPIC_v2.0/EPIC-8v2-0_A2.csv')._Manifest__data_frame + else: + manifest = Manifest(array_type, on_lambda=on_lambda, verbose=verbose)._Manifest__data_frame # 'custom', '27k', '450k', 'epic', 'epic+' LOGGER.setLevel(logging.INFO) x_probes = manifest.index[manifest['CHR']=='X'] y_probes = manifest.index[manifest['CHR']=='Y'] diff --git a/methylcheck/probes/filters.py b/methylcheck/probes/filters.py index 3702dd3..e66dabf 100644 --- a/methylcheck/probes/filters.py +++ b/methylcheck/probes/filters.py @@ -65,11 +65,13 @@ def _import_probe_exclusion_list(array, _type): Parameters ---------- - array -- [EPIC, EPIC+, 450k, 27k] or ['IlluminaHumanMethylationEPIC', 'IlluminaHumanMethylationEPIC+', 'IlluminaHumanMethylation450k'] + array -- [EPICv2, EPIC, EPIC+, 450k, 27k] or ['IlluminaHumanMethylationEPICv2', 'IlluminaHumanMethylationEPIC', 'IlluminaHumanMethylationEPIC+', 'IlluminaHumanMethylation450k'] type -- [sex, control]""" if array in ('IlluminaHumanMethylation450k','450k','450K'): filename = '450k_{0}.npy'.format(_type) + elif array in ('IlluminaHumanMethylationEPICv2','EPICv2','epicv2'): + filename = 'EPICv2_{0}.npy'.format(_type) elif array in ('IlluminaHumanMethylationEPIC','EPIC','epic'): filename = 'EPIC_{0}.npy'.format(_type) elif array in ('IlluminaHumanMethylationEPIC+','EPIC+','epic+','EPICPLUS','EPIC_PLUS'): @@ -81,7 +83,7 @@ def _import_probe_exclusion_list(array, _type): return [] else: raise ValueError("""Did not recognize array type. Please specify one of - 'IlluminaHumanMethylation450k', 'IlluminaHumanMethylationEPIC', '450k', 'EPIC', 'EPIC+', '27k', 'mouse'.""") + 'EPICv2', 'IlluminaHumanMethylation450k', 'IlluminaHumanMethylationEPIC', '450k', 'EPIC', 'EPIC+', '27k', 'mouse'.""") with resources.path(pkg_namespace, filename) as filepath: probe_list = np.load(filepath, allow_pickle=True) return probe_list @@ -94,8 +96,8 @@ def exclude_sex_control_probes(df, array, no_sex=True, no_control=True, verbose= ---------- df: dataframe of beta values or m-values. array: type of array used. - {'27k', '450k', 'EPIC', 'EPICPLUS', 'MOUSE'} or {'IlluminaHumanMethylation27k','IlluminaHumanMethylation450k','IlluminaHumanMethylationEPIC', 'mouse'} - or {'27k', '450k', 'epic', 'epic+', 'mouse'} + {'27k', '450k', 'EPIC', 'EPICPLUS', 'EPICV2, 'MOUSE'} or {'IlluminaHumanMethylation27k','IlluminaHumanMethylation450k','IlluminaHumanMethylationEPIC', 'mouse'} + or {'27k', '450k', 'epic', 'epic+', 'epicv2', 'mouse'} Optional Arguments ------------------ @@ -125,6 +127,8 @@ def exclude_sex_control_probes(df, array, no_sex=True, no_control=True, verbose= 'EPIC_PLUS': 'epic+', '450K': '450k', '27K': '27k', + 'EPICv2': 'epicv2', + 'IlluminaHumanMethylationEPICv2': 'epicv2', } if array in translate: array = translate[array] diff --git a/methylcheck/qc_plot.py b/methylcheck/qc_plot.py index b5e924f..c0c3525 100644 --- a/methylcheck/qc_plot.py +++ b/methylcheck/qc_plot.py @@ -883,6 +883,8 @@ def bis_conversion_control(path_or_df, use_median=False, on_lambda=False, verbos except ImportError: import pkg_resources pkg_namespace = 'methylcheck.data_files' + + try: with resources.path(pkg_namespace, f'{array_type}_extC.csv') as probe_filepath: ext_C_probes = pd.read_csv(probe_filepath) diff --git a/methylcheck/qc_report.py b/methylcheck/qc_report.py new file mode 100644 index 0000000..314b098 --- /dev/null +++ b/methylcheck/qc_report.py @@ -0,0 +1,657 @@ +import pandas as pd +from pathlib import Path +from io import StringIO +import re +import sys +import logging +import math +LOGGER = logging.getLogger(__name__) +# app +import methylcheck + +run_qc = methylcheck.run_qc + +__all__ = ['run_pipeline', 'run_qc'] + +def run_pipeline(df, **kwargs): + """Run a variety of probe and sample filters in tandem, then plot results + +by specifying all of your options at once, instead of running every part of methylcheck +in piacemeal fashion. + +this is analogous to using the methylcheck CLI, but for notebooks/scripts + +required: + df: (required) + - data as a DataFrame of beta values + - sample names in columns and probes in rows + +parameters: + verbose: (True/False) + default: False -- shows extra info about processing if True + silent: (True/False) + default: False -- suppresses all warnings/info + + exclude_sex: + filters out probes on sex-chromosomes + exclude_control: + filters out illumina control probes + exclude_all: + filters out the most probes (sex-linked, control, and all sketchy-listed probes from papers) + exclude: (list of strings, shorthand references to papers with sketchy probes to exclude) + + If the array is 450K the publications may include: + ``'Chen2013' + 'Price2013' + 'Zhou2016' + 'Naeem2014' + 'DacaRoszak2015'`` + If the array is EPIC the publications may include: + ``'Zhou2016' + 'McCartney2016'`` + or these reasons: + ``'Polymorphism' + 'CrossHybridization' + 'BaseColorChange' + 'RepeatSequenceElements'`` + or use ``'exclude_all'``: + to do maximum filtering, including all of these papers' lists. + + plot: (list of strings) + ['mean_beta_plot', 'beta_density_plot', 'cumulative_sum_beta_distribution', 'beta_mds_plot', 'all'] + if 'all', then all of these plots will be generated. if omitted, no plots are created. + save_plots: (True|False) + default: False + export (True|False): + default: False -- will export the filtered df as a pkl file if True + +note: + this pipeline cannot also apply the array-level methylcheck.run_qc() function + because that relies on additional probe information that may not be present. Everything + in this pipeline applies to a dataframe of beta or m-values for a set of samples. + +returns: + a filtered dataframe object + """ + # verify kwargs are expected strings + param_list ={ + 'exclude': ['Chen2013', + 'Price2013', + 'Zhou2016', + 'Naeem2014' + 'DacaRoszak2015', + 'Zhou2016', + 'McCartney2016', + 'Polymorphism', + 'CrossHybridization', + 'BaseColorChange', + 'RepeatSequenceElements'], + 'verbose': [True, False], + 'silent': [True, False], + 'exclude_sex': [True, False], + 'exclude_control': [True, False], + 'exclude_all': [True, False], + 'plot': ['mean_beta_plot', 'beta_density_plot', 'cumulative_sum_beta_distribution', 'beta_mds_plot', 'all'], + 'save_plots': [True, False], + 'export': [True, False], + 'run_qc': [True, False], + 'on_lambda': [True, False], + } + for k,v in kwargs.items(): + if k not in param_list: + raise KeyError(f"{k} not an option among {list(param_list.keys())}") + possible_values = param_list[k] + if type(v) is list: + for item in v: + if item not in possible_values: + raise ValueError(f"{item} not a valid option for {k}: {possible_values}") + else: + if v not in possible_values: + raise ValueError(f"{item} not a valid option for {k}: {possible_values}") + # now we know all inputs are among the allowed/expected params. + + # set detail level of messages + if kwargs.get('verbose') == True: + logging.basicConfig(level=logging.INFO) + else: + kwargs['verbose'] = False + logging.basicConfig(level=logging.WARNING) + if kwargs.get('silent') == True: + logging.basicConfig(level=logging.ERROR) + else: + kwargs['silent'] = False + + # assign some default pipeline params, if not specified by user + default_params = { + 'verbose': False, + 'silent': False, + 'exclude_all': True, + 'plot': 'all', + 'save_plots': False, + 'export': False, + 'on_lambda': False} + for param,setting in default_params.items(): + if kwargs.get(param) is None: + kwargs[param] = setting + + # determine array type + array_type = methylcheck.detect_array(df, on_lambda=kwargs.get('on_lambda')) + + # apply some filters + if kwargs.get('exclude_all'): + try: + df = methylcheck.exclude_sex_control_probes(df, array_type, verbose=kwargs.get('verbose')) + sketchy_probe_list = methylcheck.list_problem_probes(array_type) + df = methylcheck.exclude_probes(df, sketchy_probe_list) + except ValueError: + exc_type, exc_value, exc_traceback = sys.exc_info() + if "probe list" in str(exc_value) or "probe exclusion lists" in str(exc_value): + # keep df the same instead of filtering. + LOGGER.info(exc_value) + else: + raise Exception(exc_value) + elif kwargs.get('exclude'): + # could be a list or a string + if type(kwargs['exclude']) is not list: + kwargs['exclude'] = [kwargs['exclude']] + try: + sketchy_probe_list = methylcheck.list_problem_probes(array_type, criteria=kwargs['exclude']) + df = methylcheck.exclude_probes(df, sketchy_probe_list) + except: + exc_type, exc_value, exc_traceback = sys.exc_info() + if "probe list" in str(exc_value) or "probe exclusion lists" in str(exc_value): + LOGGER.info(exc_value) + else: + raise Exception(exc_value) + + # apply some more filters + if kwargs.get('exclude_sex') and kwargs.get('exclude_control'): + df = methylcheck.exclude_sex_control_probes(df, array_type, verbose=kwargs.get('verbose')) + elif kwargs.get('exclude_sex'): + df = methylcheck.exclude_sex_control_probes(df, array_type, no_sex=True, no_control=False, verbose=kwargs.get('verbose')) + elif kwargs.get('exclude_control'): + df = methylcheck.exclude_sex_control_probes(df, array_type, no_sex=False, no_control=True, verbose=kwargs.get('verbose')) + + # plot stuff + # run calculations and plots + if 'all' in kwargs.get('plot'): + methylcheck.mean_beta_plot(df, verbose=kwargs['verbose'], save=kwargs['save_plots'], silent=kwargs['silent']) + methylcheck.beta_density_plot(df, verbose=kwargs['verbose'], save=kwargs['save_plots'], silent=kwargs['silent']) + wide_df = df.copy().transpose() + methylcheck.cumulative_sum_beta_distribution(wide_df, verbose=kwargs['verbose'], save=kwargs['save_plots'], silent=kwargs['silent']) + pre_mds = wide_df.shape[0] + df = methylcheck.beta_mds_plot(wide_df, verbose=kwargs['verbose'], save=kwargs['save_plots'], silent=kwargs['silent']) + LOGGER.info(f"MDS filtering dropped {pre_mds - df.shape[1]} samples from dataframe.") + else: + if 'mean_beta_plot' in args.plot: + methylcheck.mean_beta_plot(df, verbose=kwargs['verbose'], save=kwargs.get('save_plots'), silent=kwargs['silent']) + if 'beta_density_plot' in args.plot: + methylcheck.beta_density_plot(df, verbose=kwargs['verbose'], save=kwargs['save_plots'], silent=kwargs['silent']) + if 'cumulative_sum_beta_distribution' in args.plot: + wide_df = df.copy().transpose() + methylcheck.cumulative_sum_beta_distribution(wide_df, verbose=kwargs['verbose'], save=kwargs['save_plots'], silent=kwargs['silent']) + if 'beta_mds_plot' in args.plot: + wide_df = df.copy().transpose() + pre_mds = wide_df.shape[0] + df = methylcheck.beta_mds_plot(wide_df, verbose=kwargs['verbose'], save=kwargs['save_plots'], silent=kwargs['silent']) + LOGGER.info(f"MDS filtering dropped {pre_mds - df.shape[1]} samples from dataframe.") + # also has filter_stdev params to pass in. + if kwargs.get('export') == True: + outfile = 'beta_values_filtered.pkl' # in long format for faster loading + df.to_pickle(outfile) + LOGGER.info(f'Saved {outfile}') + return df + + +def detection_poobah(poobah_df, pval_cutoff): + """Given a dataframe of p-values with sample_ids in columns and probes in index, + calculates the percent failed probes per sample. Part of QC report.""" + out = {} + for sample_id in poobah_df.columns: + total = poobah_df.shape[0] + failed = poobah_df[sample_id][poobah_df[sample_id] >= pval_cutoff].count() + percent_failed = round(100*failed/total,1) + out[sample_id] = percent_failed + return out + +class ReportPDF: + """ReportPDF allows you to build custom QC reports. + +------- +To use: +------- + +- First, initialize the report and pass in kwargs, like ``myReport = ReportPDF(**kwargs)`` +- Next, run ```myReport.run_qc()`` to fill it in. +- Third, you must run ``myReport.close()`` after ``run_qc()`` to save the file to disk. +- You can supply kwargs to specify which QC plots to include + - and supply a list of chart names to control the order of objects in the report. + - if you pass in 'order' in kwargs, any page you omit in the order will be omitted from the final report. + - You may pass in a custom table to override one of the built-in pages. +- include 'path' with the path to your processed pickle files. +- include an optional 'outpath' for where to save the pdf report. + +------- +kwargs: +------- + +- processing params + - filename + - poobah_max_percent + - pval_cutoff + - outpath + - path +- front page text + - title + - author + - subject + - keywords +- if 'debug=True' is in kwargs, + - then it will return a report without any parts that failed. + +-------------- +custom tables: +-------------- + +pass in arbitrary data using kwarg ``custom_tables`` as list of dictionaries with this structure: + +```python +custom_tables=[ + { + 'title': "some title, optional", + 'col_names': [""], + 'row_names': [""], + 'data': [""], + 'order_after': ["string name of the plot this should come after. It cannot appear first in list."] + }, + {"<...second table here...>"} +] +``` + +------------------------ +Pre-processing pipeline: +------------------------ + + Probe-level (w/explanations of suggested exclusions) + - Links to recommended probe exclusion lists/files/papers + - Background subtraction and normalization ('noob') + - Detection p-value ('neg' vs 'oob') + - Dye-bias correction (from SeSAMe) + Sample-level (w/explanations of suggested exclusions) + Detection p-value (% failed probes) + - custom detection (% failed, of those in a user-defined-list supplied to function) + MDS + Suggested for customer to do on their own + - Sex check + - Age check + - SNP check + """ + # larger font + # based on 16pt with 0.1 (10% of page) margins around it: use 80, 26, 16 + # MAXWIDTH = 80 # based on 16pt with 0.1 (10% of page) margins around it + # MAXLINES = 26 + # FONTSIZE = 16 + # ORIGIN = (0.1, 0.1) # achored on page in lower left + # + # normal font -- for 12pt font: use 100 x 44 lines + MAXWIDTH = 100 + MAXLINES = 44 + FONTSIZE = 12 + ORIGIN = (0.1, 0.05) # achored on page in lower left + + def __init__(self, **kwargs): + # https://stackoverflow.com/questions/8187082/how-can-you-set-class-attributes-from-variable-arguments-kwargs-in-python + self.__dict__.update(kwargs) + self.debug = True if self.__dict__.get('debug') == True else False + self.__dict__.pop('debug',None) + self.__dict__['poobah_max_percent'] = self.__dict__.get('poobah_max_percent', 5) + self.__dict__['pval_cutoff'] = self.__dict__.get('pval_cutoff', 0.05) + self.errors = self.open_error_buffer() + + #SHELVING the PDF_PART, since it worked but not neeeded right now + from matplotlib.backends.backend_pdf import PdfPages + self.outfile = Path(self.__dict__.get('outpath','.'), self.__dict__.get('filename', 'multipage_pdf.pdf')) + self.pdf = PdfPages(self.outfile) + import matplotlib.pyplot as plt + self.plt = plt + import textwrap + self.textwrap = textwrap + import datetime + self.today = str(datetime.date.today()) + self.on_lambda = self.__dict__.get('on_lambda', False) + d = self.pdf.infodict() + if any(kwarg in ('title','author','subject','keywords') for kwarg in kwargs): + # set the file's metadata via the PdfPages object: + d['Title'] = self.__dict__.get('title','') + d['Author'] = self.__dict__.get('author','') + d['Subject'] = self.__dict__.get('subject','') + d['Keywords'] = self.__dict__.get('keywords','') + d['CreationDate'] = datetime.datetime.today() + d['ModDate'] = datetime.datetime.today() + + if 'order' in self.__dict__: + self.order = self.__dict__['order'] + else: + self.order = ['beta_density_plot', 'detection_poobah', 'mds', 'auto_qc', + 'M_vs_U', 'qc_signal_intensity', 'controls', 'probe_types'] + + self.tests = { + 'detection_poobah': self.__dict__.get('poobah',True), + 'mds': self.__dict__.get('mds',True), + 'auto_qc': self.__dict__.get('auto_qc',True), # NOT IMPLEMENTED YET # + } + self.plots = { + 'beta_density_plot': self.__dict__.get('beta_density_plot',True), + 'M_vs_U': self.__dict__.get('M_vs_U',True), + 'qc_signal_intensity': self.__dict__.get('qc_signal_intensity',True), # not sure Brian wanted this, but it is available + 'controls': self.__dict__.get('controls',True), # genome studio plots + 'probe_types': self.__dict__.get('probe_types',True), + # FUTURE lower priority: bis-conversion completeness, or GCT score --- https://rdrr.io/bioc/sesame/src/R/sesame.R + } + + self.custom = {} + if 'custom_tables' in self.__dict__: + self.parse_custom_tables(self.__dict__['custom_tables']) + LOGGER.info(f"Found custom_tables and inserted into order: {self.order}") + LOGGER.info(self.custom) + + def parse_custom_tables(self, tables): + """tables is a list of { + 'title': "some title, optional", + 'col_names': [list of strings], + 'row_names': [list of strings, optional], + 'data': [list of lists, with order matching col_names], + 'order_after': [string name of the plot this should come after. It cannot appear first in list.] + }""" + for table in tables: + required_attributes = {'title', 'data', 'order_after', 'col_names'} + for i in required_attributes: + if i not in table: + raise KeyError("Your custom table must contain these keys: {required_attributes} (row_names is optional)") + #1 place order -- refer to this later in self.custom + try: + _index = self.order.index(table['order_after']) + self.order.insert(_index, table['title']) + self.custom[table['title']] = table + except ValueError: + raise ValueError(f"Your custom table's 'order_after' label is not in this list of chart objects, so could not be ordered: {self.order}") + if not isinstance(table['col_names'], list): + raise TypeError(f"Your custom table 'col_names' must be a list.") + if table.get('row_names') and not isinstance(table['row_names'], list): + raise TypeError(f"Your custom table 'row_names' must be a list.") + if not isinstance(table['data'], list): + raise TypeError(f"Your custom table 'data' must be a list of lists, matching the number of columns listed in col_names.") + if not (isinstance(table['data'][0],list) and len(table['data'][0]) == len(table['col_names'])): + raise TypeError(f"Your custom table 'data' must be a list of lists, matching the number of columns listed in col_names.") + # passes! later, the table will be looked-up by title name and fed into chart in order. + + def open_error_buffer(self): + """ preparing a stream of log messages to add to report appendix. """ + appendix = logging.getLogger() + appendix.setLevel(logging.INFO) + errors = StringIO() + error_handler = logging.StreamHandler(errors) + # %(module)s.%(funcName)s() ... %Y-%m-%d + formatter = logging.Formatter('^^^%(asctime)s - %(levelname)s - %(message)s', "%H:%M:%S") + error_handler.setFormatter(formatter) + appendix.addHandler(error_handler) + return errors + + def run_qc(self): + # load all the data from self.path; make S3-compatible too. + path = self.__dict__.get('path','') + if self.tests['detection_poobah'] is True and 'detection_poobah' in self.order: + try: + poobah_df = pd.read_pickle(Path(path,'poobah_values.pkl')) # off by default, process with --export_poobah + except FileNotFoundError: + raise FileNotFoundError(f"Could not load pickle file: 'poobah_values.pkl'. Add 'poobah=False' to inputs to turn this off.") + try: + beta_df = pd.read_pickle(Path(path,'beta_values.pkl')) + meth_df = pd.read_pickle(Path(path,'meth_values.pkl')) + unmeth_df = pd.read_pickle(Path(path,'unmeth_values.pkl')) + control_dict_of_dfs = pd.read_pickle(Path(path,'control_probes.pkl')) + LOGGER.info("Data loaded") + except FileNotFoundError: + raise FileNotFoundError("Could not load pickle files") + + ''' + self.functions = { # NOT USED -- needed to provide different conditions for each function below. # + 'detection_poobah': detection_poobah, + 'mds': methylcheck.beta_mds_plot, + 'auto_qc': None, + 'beta_density_plot': methylcheck.beta_density_plot, + 'M_vs_U': methylcheck.plot_M_vs_U, + 'controls': methylcheck.plot_controls, + 'qc_signal_intensity': methylcheck.qc_signal_intensity, + } + ''' + + for part in self.order: + if part in self.tests and self.tests[part] == True: + try: + if part == 'detection_poobah': + max_allowed = self.__dict__.get('poobah_max_percent') + sample_percent_failed_probes_dict = detection_poobah(poobah_df, pval_cutoff=self.__dict__['pval_cutoff']) + sample_poobah_failures = {sample_id: ("pass" if percent < max_allowed else "fail") for sample_id,percent in sample_percent_failed_probes_dict.items()} + #LOGGER.info(f'detection_poobah: {sample_percent_failed_probes_dict}') + LOGGER.info(f"Poobah: {len([k for k in sample_poobah_failures.values() if k =='fail'])} failure(s) out of {len(sample_poobah_failures)} samples.") + + list_of_lists = [] + for sample_id,percent in sample_percent_failed_probes_dict.items(): + row = [sample_id, percent, sample_poobah_failures[sample_id]] + list_of_lists.append(row) + self.to_table(list_of_lists, col_names=['Sample_ID', 'Percent', 'Pass/Fail'], + row_names=None, add_title='Detection Poobah') + + if part == 'mds': + LOGGER.info("Beta MDS Plot") + # ax and df_to_retain are not used, but could go into a qc chart + fig, ax, df_indexes_to_retain = methylcheck.beta_mds_plot(beta_df, silent=True, multi_params={'return_plot_obj':True, 'draw_box':True}) + self.pdf.savefig(fig) + self.plt.close() + #pretty_table = Table(titles=['Sample_ID', 'MDS Pass/Fail']) + #for sample_id in df_indexes_to_retain: + except Exception as e: + if self.debug: + LOGGER.error(f"Could not process {part}; {e}") + continue + else: + raise Exception(f"Could not process {part}; {e}") + + elif part in self.plots and self.plots[part] == True: + try: + if part == 'beta_density_plot': + LOGGER.info("Beta Density Plot") + fig = methylcheck.beta_density_plot(beta_df, save=False, silent=True, verbose=False, reduce=0.1, plot_title=None, ymax=None, return_fig=True) + self.pdf.savefig(fig) + self.plt.close() + elif part == 'M_vs_U': + LOGGER.info(f"M_vs_U plot") + fig = methylcheck.plot_M_vs_U(meth=meth_df, unmeth=unmeth_df, noob=True, silent=True, verbose=False, plot=True, compare=False, return_fig=True) + self.pdf.savefig(fig) + self.plt.close() + # if not plotting, it will return dict with meth median and unmeth median. + elif part == 'qc_signal_intensity': + LOGGER.info(f"QC signal intensity plot") + fig = methylcheck.qc_signal_intensity(meth=meth_df, unmeth=unmeth_df, silent=True, return_fig=True) + self.pdf.savefig(fig) + self.plt.close() + elif part == 'controls': + LOGGER.info(f"Control probes") + list_of_figs = methylcheck.plot_controls(control_dict_of_dfs, 'all', return_fig=True) + for fig in list_of_figs: + self.pdf.savefig(figure=fig, bbox_inches='tight') + self.plt.close('all') + elif part == 'probe_types': + LOGGER.info(f"Betas by probe type") + list_of_figs = methylcheck.plot_beta_by_type(beta_df, 'all', return_fig=True, silent=True, on_lambda=self.on_lambda) + for fig in list_of_figs: + self.pdf.savefig(figure=fig, bbox_inches='tight') + self.plt.close('all') + except Exception as e: + if self.debug: + LOGGER.error(f"Could not process {part}; {e}") + continue + else: + raise Exception(f"Could not process {part}; {e}") + + elif part in self.custom: + table = self.custom[part] + self.to_table(table['data'], col_names=table['col_names'], + row_names=table.get('row_names'), add_title=table['title']) + + + # and finally, appendix of log messages + appendix_msgs = self.errors.getvalue() + appendix_msgs = appendix_msgs.split('^^^') + # drop the trailing \n at end of each message, if present, but preserve within-line \n line breaks + appendix_msgs = [(msg[:-2] if msg[-2:] == '\n' else msg) for msg in appendix_msgs] + appendix_msgs.insert(0, 'APPENDIX I: PROCESSING MESSAGES') + total_rows = len(appendix_msgs) + last_row = 0 + while last_row <= total_rows: + rows_per_page = 26 + # pretest whether length will fit on a pgae + while True: + para_list = appendix_msgs[last_row : (last_row + rows_per_page)] + para_lengths = [len(i.split('\n')) for i in para_list] + extra_line_wraps = [max(len(self.textwrap.wrap(para, width=self.MAXWIDTH)) -1, 0) for para in para_list] + #print((sum(para_lengths) + len(para_lengths) + sum(extra_line_wraps))) + if (sum(para_lengths) + len(para_lengths) + sum(extra_line_wraps)) > self.MAXLINES: + rows_per_page -= 2 + #print('---',rows_per_page) + else: + break + if rows_per_page <= 0: + break + if rows_per_page <= 0: + LOGGER.error("couldn't write log messages to appendix") + break + self.page_of_paragraphs(para_list, self.pdf, line_height='single') + last_row += rows_per_page + self.errors.close() + + + def exec_summary(self): + """QC exec summary + sample_name/ID + probe % failures + probe_failure pass + auto-qc result (only if present in kwargs passed in, otherwise omitted) + MDS pass + signal intensity pass + if any fails, fail it (so overall pass) + table 2: meta + array type (detect from data) + number of samples (from data) + processing pipeline version number (passed in) + date processed (passed in) + avg probe failure rate + percent of samples that failed + any failures from 'control probes' + reqs a way to capture warnings of data-off-chart + """ + exec_summary_1 = [] + exec_summary_2 = [] # list of lists + exec_summary_samples = [] # temp storage lookup + pass + + def page_of_text(self, text, pdf): + """text is a single big string of text, with whitespace for line breaks. + https://matplotlib.org/3.1.1/api/_as_gen/matplotlib.pyplot.text.html (0,0) is lower left; (1,1) is upper right """ + print([len(i.split('\n')) for i in [text]]) + firstPage = self.plt.figure(figsize=(11,8.5)) + firstPage.clf() + wrapped_txt = self.textwrap.fill(text, width=self.MAXWIDTH) + #wrapped_txt_list = textwrap.wrap(txt, width=80) + # next, identify paragraph breaks here as strings of writespace, and wrap each part separately? then move onto page. + #firstPage.text(0.5,0.5,txt, size=12, ha="left") # transform=firstPage.transFigure + firstPage.text(self.ORIGIN[0], self.ORIGIN[1], wrapped_txt, size=self.FONTSIZE) + pdf.savefig() + self.plt.close() + + + def page_of_paragraphs(self, para_list, pdf, line_height='double'): + """ https://matplotlib.org/3.1.1/api/_as_gen/matplotlib.pyplot.text.html (0,0) is lower left; (1,1) is upper right. + this version estimates the size of each paragraph and moves the origin downward accordingly. + tricky because the anchors are lower left, not upper left. + + ok if a paragraph contains whitespace line breaks, OR each para is one long line to be wrapped here. + also - if a paragraph wraps, this accounts for it in total lines count""" + #1 - finding the start points for each paragraph in page, counting backwards, so first paragraph is at top of page. + para_lengths = [len(i.split('\n')) for i in para_list] + extra_line_wraps = [max(len(self.textwrap.wrap(para, width=self.MAXWIDTH)) -1, 0) for para in para_list] + if (sum(para_lengths) + len(para_lengths) + sum(extra_line_wraps)) > self.MAXLINES: + raise ValueError(f"Text lines {(sum(para_lengths) + len(para_lengths) + sum(extra_line_wraps))} > {self.MAXLINES}, the max lines per PDF page allowed.") + #print(para_lengths) -- ok if a paragraph contains whitespace line breaks, OR each para is one long line to be wrapped here. + # assume each line height is 0.1/26 fraction of total page height + + # paragraph spacing + if line_height == 'double': + line_height = round((1 - 2*self.ORIGIN[1])/self.MAXLINES,3) + elif line_height == 'single': + line_height = round(0.5*(1 - 2*self.ORIGIN[1])/self.MAXLINES,3) + else: + pass + # else, line_height is whatever int/float you pass in, for custom spacing. (as fraction of page, so use 0.01 to 0.03) + + paragraph_spacing = line_height # 1 line + + firstPage = self.plt.figure(figsize=(11,8.5)) + firstPage.clf() + current_y_position = (1.0 - self.ORIGIN[1]) # working down page by subtracting + extra_lines = [] # if something doesn't fit on page, include this in error message + for nth, para in enumerate(para_list): + # each para is assumed to be one paragraph of text with no whitespace formatting. + wrapped_txt = self.textwrap.fill(para, width=self.MAXWIDTH) + wrapped_addtl_line_count = max(len(self.textwrap.wrap(para, width=self.MAXWIDTH)) -1, 0) + x_margin = self.ORIGIN[0] + current_y_position -= (line_height * len(para.split('\n'))) + current_y_position -= (line_height * wrapped_addtl_line_count) + if nth > 0: + current_y_position -= paragraph_spacing + if current_y_position < self.ORIGIN[1]: + extra_lines.append(wrapped_txt) + #print(current_y_position, wrapped_addtl_line_count, wrapped_txt) + else: + firstPage.text(x_margin, current_y_position, wrapped_txt, size=self.FONTSIZE) + if extra_lines != []: + print("WARNING: DID NOT FIT") + for extra_line in extra_lines: + print(extra_line) + print("") + pdf.savefig() + self.plt.close() + + def to_table(self, list_of_lists, col_names, row_names=None, add_title=''): + """ + - embeds a table in a PDF page. + - attempts to split long tables into multiple pages. + - should warn if table is too wide to fit. """ + + # stringify numbers; replace None + for idx, sub in enumerate(list_of_lists): + list_of_lists[idx] = [('' if isinstance(i,type(None)) else str(i)) for i in sub] + + rows_per_page = 42 # appears to work for standard default PDF page size and font size. + pages = math.ceil(len(list_of_lists)/rows_per_page) + #paginate table + for page in range(pages): + #LOGGER.debug(f'page {page+1} -- {(page * rows_per_page)}:{(page + 1) * rows_per_page}') + page_data = list_of_lists[(page * rows_per_page) : (page + 1) * rows_per_page] + fig, ax = self.plt.subplots(1, figsize=(10,8)) + #ax.axis('tight') + ax.axis('off') + matplotlib_table = ax.table( + cellText=page_data, + colLabels=col_names, + loc='center', # if len(page_data) >= rows_per_page else 'top', + edges='open', + colLoc='right', + ) + matplotlib_table.auto_set_font_size(False) + matplotlib_table.set_fontsize(12) + if page == 0: + self.plt.title(add_title, y=1.1) #pad=20) # -- placement is off + self.pdf.savefig(fig) + self.plt.close() diff --git a/methylcheck/rawQC.py b/methylcheck/rawQC.py new file mode 100644 index 0000000..3f7e01d --- /dev/null +++ b/methylcheck/rawQC.py @@ -0,0 +1,165 @@ +# -*- coding: utf-8 -*- +import pkg_resources +import pandas as pd + + +def _import_probe_filter_list(array): + """Function to identify array type and import the + appropriate probe exclusion dataframe""" + + if array == 'IlluminaHumanMethylation450k': + path = 'data_files/450k_polymorphic_crossRxtve_probes.csv.gz' + filepath = pkg_resources.resource_filename(__name__, path) + filter_options = pd.read_csv(filepath) + elif array == 'IlluminaHumanMethylationEPIC': + path = 'data_files/EPIC_polymorphic_crossRxtve_probes.csv.gz' + filepath = pkg_resources.resource_filename(__name__, path) + filter_options = pd.read_csv(filepath) + else: + raise ValueError( + "Did not recognize array type. Please specify 'IlluminaHumanMethylation450k' or 'IlluminaHumanMethylationEPIC'.") + return + return filter_options + + +def createProbeExclusionList(array, pubs=None, criteria=None, custom_list=None): + """Function to create list of probes to exclude from downstream processes. + By default, all probes that have been noted in the literature to have + polymorphisms, cross-hybridization, repeat sequence elements and base color + changes are included in the exclusion list. Setting any of the args for these + reasons or publications (described below) to False will result in these probes + NOT being included in the final exclusion list.to User also has ability to + add custom list of probes to include in final returned list. + + Parameters + ---------- + array: dataframe + Dataframe containing beta values + + pubs: list + List of the publications to use when excluding probes. + If the array is 450K the publications may include: + 'Chen2013' + 'Price2013' + 'Zhou2016' + 'Naeem2014' + 'DacaRoszak2015' + If the array is EPIC the publications may include: + 'Zhou2016' + 'McCarthey2016' + If no publication list is specified, probes from + all publications will be added to the exclusion list. + If more than one publication is specified, all probes + from all publications in the list will be added to the + exclusion list. + + criteria: list + List of the criteria to use when excluding probes. + List may contain the following exculsion criteria: + 'Polymorphism' + 'CrossHybridizing' + 'BaseColorChange' + 'RepeatSequenceElements' + If no criteria list is specified, all critera will be + excluded. If more than one criteria is specified, + all probes meeting any of the listed criteria will be + added to the exclusion list. + + custom_list: list, default None + User provided list of probes to add to probe + exclusion list + + Returns + ------- + probe_exclusion_list: list + List containing probe identifiers to be excluded + + """ + probe_dataframe = _import_probe_filter_list(array) + args = [Polymorphism, CrossHybridization, + BaseColorChange, RepeatSequenceElements, + Chen2013, Price2013, Zhou2016, Naeem2014, + DacaRoszak2015, McCartney2016] + if custom_list is None: + custom_list = [] + if False not in args: + probe_exclusion_list = list(set(probe_dataframe['Probe'].values)) + probe_exclusion_list = list(set(probe_exclusion_list + custom_list)) + else: + probe_exclusion_list = [] + if Polymorphism is True: + df = probe_dataframe[probe_dataframe['Reason'] == 'Polymorphism'] + probe_exclusion_list = list( + set(df['Probe'].values)) + probe_exclusion_list + if CrossHybridization is True: + df = probe_dataframe[probe_dataframe['Reason'] + == 'CrossHybridization'] + probe_exclusion_list = list( + set(df['Probe'].values)) + probe_exclusion_list + if BaseColorChange is True: + df = probe_dataframe[probe_dataframe['Reason'] + == 'BaseColorChange'] + probe_exclusion_list = list( + set(df['Probe'].values)) + probe_exclusion_list + if RepeatSequenceElements is True: + df = probe_dataframe[probe_dataframe['Reason'] + == 'RepeatSequenceElements'] + probe_exclusion_list = list( + set(df['Probe'].values)) + probe_exclusion_list + if Chen2013 is True: + if array == 'IlluminaHumanMethylationEPIC': + raise ValueError( + "Citation Chen2013 does not exist for 'IlluminaHumanMethylationEPIC'.") + return + else: + df = probe_dataframe[probe_dataframe['ShortCitation'] + == 'Chen2013'] + probe_exclusion_list = list( + set(df['Probe'].values)) + probe_exclusion_list + if Price2013 is True: + if array == 'IlluminaHumanMethylationEPIC': + raise ValueError( + "Citation Price2013 does not exist for 'IlluminaHumanMethylationEPIC'.") + return + else: + df = probe_dataframe[probe_dataframe['ShortCitation'] + == 'Price2013'] + probe_exclusion_list = list( + set(df['Probe'].values)) + probe_exclusion_list + if Naeem2014 is True: + if array == 'IlluminaHumanMethylationEPIC': + raise ValueError( + "Citation Naeem2014 does not exist for 'IlluminaHumanMethylationEPIC'.") + return + else: + df = probe_dataframe[probe_dataframe['ShortCitation'] + == 'Naeem2014'] + probe_exclusion_list = list( + set(df['Probe'].values)) + probe_exclusion_list + if DacaRoszak2015 is True: + if array == 'IlluminaHumanMethylationEPIC': + raise ValueError( + "Citation DacaRoszak2015 does not exist for 'IlluminaHumanMethylationEPIC'.") + return + else: + df = probe_dataframe[probe_dataframe['ShortCitation'] + == 'DacaRoszak2015'] + probe_exclusion_list = list( + set(df['Probe'].values)) + probe_exclusion_list + if Zhou2016 is True: + df = probe_dataframe[probe_dataframe['ShortCitation'] + == 'Zhou2016'] + probe_exclusion_list = list( + set(df['Probe'].values)) + probe_exclusion_list + if McCartney2016 is True: + if array == 'IlluminaHumanMethylation450k': + raise ValueError( + "Citation McCartney2016 does not exist for 'IlluminaHumanMethylationEPIC'.") + return + else: + df = probe_dataframe[probe_dataframe['ShortCitation'] + == 'McCartney2016'] + probe_exclusion_list = list( + set(df['Probe'].values)) + probe_exclusion_list + probe_exclusion_list = list(set(probe_exclusion_list + custom_list)) + return probe_exclusion_list diff --git a/methylcheck/reports/bead_array.py b/methylcheck/reports/bead_array.py index a81c3ed..8c2c35d 100644 --- a/methylcheck/reports/bead_array.py +++ b/methylcheck/reports/bead_array.py @@ -103,7 +103,7 @@ class ControlsReporter(): 'Predicted Sex'] def __init__(self, filepath, outfilepath=None, bg_offset=3000, cutoff_adjust=1.0, colorblind=False, - roundoff=2, legacy=False, pval=True, pval_sig=0.05, passing=0.7, project_name=None): + roundoff=2, legacy=False, pval=True, pval_sig=0.05, passing=0.7, project_name=None, array_type=None): self.filepath = filepath # folder with methyprep processed data self.bg_offset = bg_offset self.cut = cutoff_adjust # for adjusting minimum passing per test @@ -113,6 +113,7 @@ def __init__(self, filepath, outfilepath=None, bg_offset=3000, cutoff_adjust=1.0 self.pval_sig = pval_sig # significance level to define a failed probe self.passing = passing # fraction of tests that all need to pass for sample to pass self.project_name = project_name # used to name the QC report, if defined. + self.array_type = array_type # used to determine which manifest is used, specially for epicv2 # if outfilepath is not provided, saves to the same folder where the pickled dataframes are located. if not outfilepath: self.outfilepath = filepath @@ -665,7 +666,7 @@ def process(self): LOGGER.info("Predicting Sex...") try: #print(self.noob_meth.shape, self.noob_unmeth.shape) - sex_df = methylcheck.get_sex((self.noob_meth, self.noob_unmeth), array_type=None, verbose=False, plot=False, on_lambda=False, median_cutoff= -2, include_probe_failure_percent=False) + sex_df = methylcheck.get_sex((self.noob_meth, self.noob_unmeth), array_type=self.array_type, verbose=False, plot=False, on_lambda=False, median_cutoff= -2, include_probe_failure_percent=False) except ValueError as e: if str(e).startswith('Unsupported Illumina array type'): # happens with some mouse array versions, but not fatal to the report @@ -875,7 +876,7 @@ def c(col): # converts to an EXCEL COLUMN name, like A or AE worksheet.set_column(this_box, col_width) col += 1 worksheet.set_column("A:A", 24) # samples - writer.save() + writer.close() def controls_report(*args, **kwargs): @@ -926,7 +927,10 @@ def controls_report(*args, **kwargs): if len(args) == 1: kwargs['filepath'] = args[0] LOGGER.warning(f"Assigned argument 0 `{kwargs['filepath']}` to `filepath` kwarg. This function takes 0 arguments.") - elif len(args) > 1: + elif len(args) == 2: + kwargs['filepath'] = args[0] + kwargs['array_type'] = args[1] + elif len(args) > 2: raise AttributeError("This function takes 0 arguments, but {len(args)} arguments found. Specify the filepath using filepath=.") if 'filepath' not in kwargs: raise FileNotFoundError("No control probes file location specified.") diff --git a/methylcheck/samples/assign.py b/methylcheck/samples/assign.py new file mode 100644 index 0000000..67b04af --- /dev/null +++ b/methylcheck/samples/assign.py @@ -0,0 +1,185 @@ +import pandas as pd +import numpy as np +import seaborn as sns +import matplotlib.pyplot as plt +from collections import Counter +import methylcheck + +## TODO: assign() and plot_assigned_groups() has no unit tests + +def assign(df, two_pass=False): + """Manually and interactively assign each sample to a group, based on beta-value distribution shape. + +how: + sorts samples by the position of their peak intensity in beta dist, then lets the human assign a number to + each. + assumes that peaks that differ by > 0.1 of the 0 to 1 range are different clusters. fills in expected cluster #. + +options: + if two_pass==True: it lets user go through every cluster a second time and split larger clusters further. + sometimes a cluster isn't obviously two groups until it is separated from the larger dataset. + """ + # calculate the highest bin of the sample beta histogram for each sample + def calc_max_bin_of_hist(nparray): + hist_vals, bin_edges = np.histogram(nparray, bins=16) + # want the bin_edge corresponding to the max hist_val + bin_of_max_val = np.where(hist_vals == np.amax(hist_vals)) + return bin_of_max_val[0][0] # it could occur in two bins; if so, return the first bin only + colors = {-1:"xkcd:scarlet", 2:'xkcd:brick red', 1:"xkcd:blue", 2:"xkcd:green", 3:'xkcd:gold', 4:'xkcd:purple', 5:'xkcd:grey blue', + 6:'xkcd:brown', 7:'xkcd:bubblegum', 8:'xkcd:tangerine', 9:'xkcd:poop', 10:"xkcd:baby blue", 11:"xkcd:violet", 12:"xkcd:apple green", + 13:"xkcd:neon pink", 14:"xkcd:deep green", 15:"xkcd:blush", 16:"xkcd:lemon"} + def sample_plot_color_clusters(df, colormap=None, verbose=False, save=False, silent=False, reduce=0.01, ymax=5, figsize=(14,8)): + """ colormap: dict of sample_ids --> group number (some int). Sample_ids must match df columns""" + #qualitative_colors = sns.color_palette("Set3", 10) + #sns.set_color_codes() + # ensure sample ids are unique + if df.shape[0] > df.shape[1]: + df = df.transpose() + # must have samples in rows/index to reindex. you can't "recolumn". + if list(df.index) != list(set(df.index)): + LOGGER.info("Your sample ids contain duplicates.") + # rename these for this plot only + df['ids'] = df.index.map(lambda x: x + '_' + str(int(1000000*np.random.rand()))) + df = df.set_index('ids', drop=True) + df = df.transpose() + + if df.shape[0] < df.shape[1]: + ## ensure probes in rows and samples in cols + df = df.transpose() + if reduce != None and reduce < 1.0: + if not isinstance(reduce, (int, float)): + try: + reduce = float(reduce) + except Exception as e: + raise ValueError(f"{reduce} must be a floating point number between 0 and 1.0") + probes = np.random.choice(df.shape[0], int(reduce*df.shape[0])) + fig, ax = plt.subplots(figsize=figsize) #was (12, 9) + for idx, sample_id in enumerate(df.columns): # samples + if sample_id != 'Name': # probe name + if reduce: + values = df[sample_id].values[probes] + else: + values = df[sample_id].values + if len(values.shape) > 1: + raise ValueError("Your df probaby contains duplicate sample names.") + + this_color = colors[-1] if colormap is None else colors.get(colormap.get(sample_id,-1),'k') + if idx+1 == len(df.columns): + kde_kws = {'linestyle':'--', 'linewidth':2} + else: + kde_kws = {'linewidth':1} + + sns.distplot( + values, hist=False, rug=False, kde=True, + ax=ax, axlabel='beta', color=this_color, kde_kws=kde_kws) + (data_ymin,data_ymax) = ax.get_ylim() + if data_ymax > ymax: + ax.set_ylim(0,ymax) + plt.legend(bbox_to_anchor=(1.05, 1), loc=2, borderaxespad=0.) + plt.show() + + # orient so samples in columns + if len(df.columns) > df.shape[0]: + df = df.transpose() # now more rows(probes) than cols(samples) + + def color_guide(user_defined_groups): + color_names = {k: v.replace('xkcd:','') for k,v in colors.items()} + color_count = Counter(user_defined_groups.values()) + guide = [] + for group,count in color_count.most_common(): + guide.append(f"{group} {color_names[group]}") + return ' | '.join(guide) + + sample_predicted_groups = {} # sample_name --> bin where peak occurs | as a 0-10 number for clustering + for sample_id in df.columns: # columns + #betas[sample] = vectorized_func(meth, unmeth) + sample_predicted_groups[sample_id] = calc_max_bin_of_hist(df[sample_id].values) + print('Predicted sample groups, based on beta-peak position') + prediction_note = " | ".join([f"{k}: {v}" for k,v in Counter(sample_predicted_groups.values()).most_common()]) + print(prediction_note) + # sort by cluster frequency + sample_predicted_groups_counts = Counter(sample_predicted_groups.values()) + sample_predicted_groups = dict(sorted(sample_predicted_groups.items(), key=lambda kv: sample_predicted_groups_counts[kv[1]], reverse=True)) + + # go through each sample, plotting it, and letting user assign to one of the groups + user_defined_groups = {} + colormap = {} + past_samples = [] + for sample_id,predicted_group in sample_predicted_groups.items(): + past_samples.append(sample_id) + sub_sample_df = pd.DataFrame(data = df[past_samples], index = df.index).transpose() + sample_plot_color_clusters(sub_sample_df, colormap=colormap, reduce=0.01) + print(color_guide(user_defined_groups)) + while True: + g = input(f"Group ({predicted_group}): ") + if g in (""," "): + g = predicted_group + try: + g = int(g) + except: + print('Only use numbers between -1 and 16!') + g = input(f"Group ({predicted_group}): ") + if g not in colors: + print('Only use numbers between -1 and 16!') + g = input(f"Group ({predicted_group}): ") + else: + break + user_defined_groups[sample_id] = g + if g == -1: + g = 10 + colormap[sample_id] = g + + # finally, produce a table and plot of each cluster: + clusters = list(set(user_defined_groups.values())) + cluster_samples = {} + for cluster in clusters: + samples = [sample_id for sample_id in df.columns if user_defined_groups.get(sample_id) == cluster] + sub_sample_df = pd.DataFrame(data = df[samples], index = df.index) + print(f"Group ({cluster}) {len(samples)} samples: {samples}") + methylcheck.sample_plot(sub_sample_df) + if two_pass: + yesno = input(f"Rework Group ({cluster}) (y/n)?") + if yesno in ('y','Y','yes'): + past_samples = [] + for sample_id in samples: + past_samples.append(sample_id) + sub_sample_df = pd.DataFrame(data = df[past_samples], index = df.index).transpose() + sample_plot_color_clusters(sub_sample_df, colormap=colormap, reduce=0.01) + print(color_guide(user_defined_groups)) + while True: + g = input(f"Group ({cluster}): ") + if g in (""," "): + g = cluster + try: + g = int(g) + except: + print('Only use numbers between -1 and 16!') + g = input(f"Group ({cluster}): ") + if g not in colors: + print('Only use numbers between -1 and 16!') + g = input(f"Group ({cluster}): ") + else: + break + user_defined_groups[sample_id] = g + if g == -1: + g = 10 + samples = [sample_id for sample_id in df.columns if user_defined_groups.get(sample_id) == cluster] + sub_sample_df = pd.DataFrame(data = df[samples], index = df.index) + print(f"Group ({cluster}) {len(samples)} samples: {samples} REVISED") + methylcheck.sample_plot(sub_sample_df) + cluster_samples[cluster] = samples + return user_defined_groups + +def plot_assigned_groups(df, user_defined_groups): + """ takes the 'sample: group' dictionary and plots each sub-set. + returns a lookup dict of each cluster --> [list of samples].""" + # finally, produce a table and plot of each cluster: + clusters = list(set(user_defined_groups.values())) + cluster_samples = {} + for cluster in clusters: + samples = [sample_id for sample_id in df.columns if user_defined_groups.get(sample_id) == cluster] + sub_sample_df = pd.DataFrame(data = df[samples], index = df.index) + plot_title = f"Group ({cluster}) {len(samples)} samples" + methylcheck.beta_density_plot(sub_sample_df, plot_title=plot_title) + cluster_samples[cluster] = samples + return cluster_samples