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Hi, @williamstark01, when I implement normalizing the label, the labels are represented by a tuple(left, right), and the original sequence(seqstart: seqend) should convert into the range from 0 to 1. So, if in the tuple, the sequence should be ((left - seqstart) / (seqstart - seqend), (right - seqend) / (seqstart - seqend)). In this procedure, we should concern with the double strands, the forward strand has a situation that seqstart < seqend, and the reverse strand has the opposite property. So, two types of strands will feed into our model, and our model will have to produce its own output types, it will require our model have the power of identifying the two types. It will be a burden for our model. We should promise our model there is only one type of strand will be the input. There is a workaround every reverse strand can be converted to a forward strand, so everything will be solved, also, to quickly prove our concept of DETR in biology, we can temporarily ignore the reverse strand. what do you think about this? 😃