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nextflow_schema.json
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{
"$schema": "https://json-schema.org/draft/2020-12/schema",
"$id": "https://raw.githubusercontent.com/elembio/base2fastq/master/nextflow_schema.json",
"title": "elembio/base2fastq pipeline parameters",
"description": "FASTQ generation using Bases2Fastq for Element AVITI System",
"type": "object",
"$defs": {
"input_output_options": {
"title": "Input/output options",
"type": "object",
"fa_icon": "fas fa-terminal",
"description": "Define where the pipeline should find input data and save output data.",
"required": ["run_dir", "outdir"],
"properties": {
"run_dir": {
"type": "string",
"description": "Input run directory"
},
"outdir": {
"type": "string",
"format": "directory-path",
"description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.",
"fa_icon": "fas fa-folder-open",
"default": "."
},
"run_manifest": {
"type": "string",
"description": "(optional) path to custom RunManifest.csv"
}
}
},
"bases2fastq": {
"title": "Bases2Fastq",
"type": "object",
"description": "",
"default": "",
"properties": {
"legacy_fastq": {
"type": "boolean",
"description": "Apply a legacy file naming convention to FASTQ files, such as SampleName_S1_L001_R1_001.fastq.gz. Some secondary analysis software might require this naming convention."
},
"detect_adapters": {
"type": "boolean",
"description": "Detect adapter sequences and override any sequences present in the run manifest."
},
"force_index_orientation": {
"type": "boolean",
"description": "Perform demultiplexing without detecting the index sequence orientation. When used, Bases2Fastq applies the orientation recorded in the run manifest."
},
"num_unassigned": {
"type": "number",
"description": "Indicates the maximum number of unassigned sequences to support.",
"minimum": 1,
"maximum": 1000
},
"qc_only": {
"type": "boolean",
"description": "Enable QC-only mode, which generates a representative view of run metrics on one tile and no FASTQ files. Executions in QC-only mode are faster than executions with demultiplexing."
},
"split_lanes": {
"type": "boolean",
"description": "Divide FASTQ files by flow cell lane."
},
"settings": {
"type": "string",
"default": "[]",
"description": "Overwrite run manifest settings. May be applies more than once."
},
"flowcell_id": {
"type": "string",
"description": "Overwrite the flow cell ID in RunParameters.json with another flow cell ID. You can use this argument to add a missing flow cell ID."
},
"filter_mask": {
"type": "string",
"description": "\"Apply a custom base mask to specify the cycles that determine if a read passes filter. Separate each read section with a hyphen. The argument is only compatible with Cloudbreak or Cloudbreak Freestyle chemistry"
}
}
},
"bases2fastq_advanced_settings": {
"title": "Bases2Fastq Advanced Settings",
"type": "object",
"description": "",
"default": "",
"properties": {
"exclude_tile": {
"type": "string",
"default": "[]",
"description": "Specify subset of tiles to exclude from processing. Use a regex to identify the tiles (e.g. 'L1R..C..S.')"
},
"include_tile": {
"type": "string",
"default": "[]",
"description": "Specify subset of tiles to include in processing. Use a regex to identify the tiles (e.g. 'L1R..C..S.'). To include specific tiles, you must first exclude all tiles with the exclude-tile parameter"
},
"no_error_on_invalid": {
"type": "boolean",
"description": "Skip non-critical files that Bases2Fastq identifies as invalid"
},
"b2f_args": {
"type": "string",
"description": "Send a string of arguments to Bases2Fastq. Recommended for local testing & development only."
}
}
},
"infrastructure": {
"title": "Infrastructure",
"type": "object",
"description": "",
"default": "",
"properties": {
"max_cpus": {
"type": "integer",
"default": 48
},
"max_memory": {
"type": "string",
"default": "192.GB"
},
"max_time": {
"type": "string",
"default": "8.h"
},
"b2f_container_url": {
"type": "string",
"default": "docker.io/elembio/bases2fastq"
},
"b2f_container_tag": {
"type": "string",
"default": "latest"
},
"tracedir": {
"type": "string",
"default": "./pipeline_info"
},
"enable_conda": {
"type": "boolean"
},
"publish_dir_mode": {
"type": "string",
"default": "copy"
},
"custom_config_version": {
"type": "string",
"default": "master"
},
"custom_config_base": {
"type": "string",
"default": "https://raw.githubusercontent.com/nf-core/configs/master"
},
"config_profile_description": {
"type": "string"
},
"config_profile_contact": {
"type": "string"
},
"config_profile_url": {
"type": "string"
},
"config_profile_name": {
"type": "string"
}
}
},
"multiqc": {
"title": "MultiQC",
"type": "object",
"description": "",
"default": "",
"properties": {
"multiqc_title": {
"type": "string"
},
"max_multiqc_email_size": {
"type": "string",
"default": "25.MB"
},
"multiqc_logo": {
"type": "string"
},
"multiqc_config": {
"type": "string"
}
}
}
},
"allOf": [
{
"$ref": "#/$defs/input_output_options"
},
{
"$ref": "#/$defs/bases2fastq"
},
{
"$ref": "#/$defs/bases2fastq_advanced_settings"
},
{
"$ref": "#/$defs/infrastructure"
},
{
"$ref": "#/$defs/multiqc"
}
],
"properties": {
"projects": {
"type": "boolean",
"default": true
}
}
}