I know that readlif has the access to .lif file. When I try to use numpy to process the readout of .lif file, I find it is different from the LAS X navigator.
Below is the image of LAS X, which is setting the 1st channel with Z projection (MAX intensity) method.

And below is the image I got with readlif and numpy.

The spot is similar but the details in the pixel are different.