Prostate cancer WSIs datasets #7
Description
Is the Prostate cancer WSIs in the manuscript your own dataset or public data? If it is public data, can you provide a link to the Prostate cancer WSIs data?
The description of Prostate cancer WSIs in the manuscript is as follows:
Prostate cancer WSIs. Primary stage prostate cancer FFPE tissue sections (4 μm) were deparaffinized and used for heat-mediated antigen retrieval (citrate buffer, pH 6, Vector Labs). Sections were blocked for 10 min in 3% H2O2, followed by 30 min room temperature incubation in 1% bovine serum albumin in phosphate-buffered saline–0.1% Tween 20. The following primary antibodies were used: anti-CD146 (Abcam, catalogue no. ab75769, EPR3208, 1:500), anti-AR (Abcam, catalogue no. ab133273, EPR1535, 1:100) and anti-NKX3.1 (Cell Signaling, catalogue no. 83700T, D2Y1A, 1:200). Secondary anti-rabbit antibody Envision horseradish peroxidase (DAKO, Agilent Technologies, catalogue no. K400311-2, undiluted) was incubated for 30 min, and signal detection was done using 3-amino-9-ethylcarbazole substrate (DAKO, Agilent Technologies). Sections were counterstained with hematoxylin and mounted with aquatex. Images were acquired using a 3D Histech Panoramic Flash II 250 scanner at ×20 magnification (resolution 0.24 μm per pixel).